厌氧菌酶动力学变化可能与细菌的代谢有关

比较分析牙龈卟啉菌和牙髓卟啉菌之间的代谢特征以及与细菌酶动力学变化的关系，并浴细菌的外膜成分观察了细菌生长过程中的形态学变化。方法：提取Ｐｇ，Ｐｅ的外膜成分和培养上清，采用厌氧菌生长率测定和酶动力学测定法。结论：Ｐｅ可能对分解代谢所形成的产物的反馈抑制作较为敏感，显示Ｐｇ对这种反馈抑制的耐受能力较强。

Enzymological kinetics of anaerobes might have relevance to the bacterial metabolism

Obiective: To compare and analyze the relationship of the metabolistic properties of both P. gingivalis (Pg) and P. endodontalis (Pe) with their characteraization of enzymic kinetics. Methods: Extracellular vesicles (EV), outer membrane protein complex (OMPC) and EV free supernatant (VS) of Pg W50 and Pe ATCC35406 were isolated with salt precipitation and studied with bacterial growth ratio test and enzymic kinetic examination. Results: Both strains grew in logarithemic way during the first three or four days. The change of enzymic activities of Pe was greater than that of Pg. Release of the enzyme which mainly located in cellular components (bacterial cell, OMPC, EV) of Pe into surrounding micro environment was earlier than Pg. With continual culture, the supernatant would gradually be the locality of most enzymic activities. Conclusion: The difference in distribution of enzymological kinetics might reflect the diversity of capability of carrying the toxic factors on the outer membrane of bacteria and Pg might have higher endurance to feedback inhibitive substances.