Immunosurveillance modelled in vitro: naive and memory T cells spontaneously migrate across unstimulated microvascular endothelium |
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Authors: | Rohnelt RK; Hoch G; Reiss Y; Engelhardt B |
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Institution: | Max-Planck-Institut fur physiologische, Abt. Molekulare Zellbiologie, Bad Nauheim, Germany. |
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Abstract: | As a model for T cell immigration into non-lymphoid tissue we set up an in
vitro assay that would allow us to investigate the phenotype of T
lymphocytes from peripheral lymph nodes (PLN), mesenteric lymph nodes (MLN)
or peripheral blood (PBL) of mice, which were able to spontaneously migrate
across unstimulated microvascular endothelium. The transendothelial
migrating T cell population was enriched for T lymphocytes expressing a
"recently activated/memory' phenotype: LFA- 1/CD44/ICAM-1high, but also
contained CD45RBhigh and LFA-1low T cells, which in the case of MLN T cells
were phenotyped as CD4+ and thus characterized as naive T cells.
Transmigrated T cells could be further distinguished from their original
populations and from each other by their distinct but heterogeneous
expression patterns for L-selectin, alpha 4 beta 7-integrin and PECAM-1.
This observation suggests the presence of phenotypically different
migratory T cells among MLN, PLN and PBL. Additional studies provided
evidence that the capacity to migrate across unstimulated microvascular
endothelium was a characteristic of a T cell population that could
phenotypically be differentiated from activated T cells. The endothelial
cells were found to play an active role in selecting the traversing T cell
population, as they controlled the number and phenotype of spontaneously
transmigrating T cells. Our studies suggest that the capacity to
transmigrate across unstimulated microvascular endothelium and hence to
immigrate into non-lymphoid tissue is owned by a phenotypically
heterogeneous T cell population, which is enriched for memory T cells but
not devoid of naive T cells.
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