80例狼疮肾炎病人ANA荧光核型及抗核抗体谱分析

目的探讨抗核抗体（ANA）荧光核型及抗核抗体谱多肽谱对狼疮性肾炎（LN）诊断的有效指标。方法对80例LN组、78例系统性红斑狼疮（SLE）非LN组及104例非SLE组分别用间接荧光法（IIF—ANA）和线性免疫印迹法（uA—ANA）进行抗核抗体荧光核型、双链DNA及11项抗核抗体谱多肽谱分析。结果LN组抗核抗体阳性率（〉1：100）为100％（80／80），LN组荧光核型均质型42．5％（34／80）显著高于非IN组21．79％（17／78）与对照组5．77％（6／104），P〈0．05；LN组颗粒核型53．75％（43／80）明显低于非LN组73．08％（57／78），P〈0．05。LN病例核均质型主要表现有抗Hi、Nud、dsDNA抗体，x2为8．26、23．05、26．39（e均〈0．05）。抗核抗体谱多肽谱分析，LJN组仅抗dsDNA抗体55．0I）％（44／80）高于非LN组23．08％（18／78），差异有显著性，P〈0．C15。结论SLE伴狼疮性肾炎的病人ANA荧光核型主要表现为均质型，抗dsDNA抗体阳性率显著性增高，可视为预测狼疮性肾炎的有效指标。

Analysis of Anti-nuclear Antibody Fluorescence Patterns and ExtractableNuclear Antibody for 80 Patients with Lupus nephritis

Objective To discuss the diagnostic effective indicators of anti-nuclear antibody（ANA） fluorescence pat- terns and extractable nuclear antibody（ENA） for the patients with lupus nephritis （LN）. Methods We enrolled 158 sys- temic lupus （SLE） patients,of whom 80 were diagnosed with LN, the other 78 were not accompanied with LN, 104 people without SLE were regarded as control group. Autoantibodies were measured and analyzed in 158 SLE patients and control group. The levels of ANA and anti-double stranded DNA（anti-dsDNA） antibody were measured by indirect immunofluoromet- ric assay,and other 11 anti-ENA antibody were determined by linearity immunoblotting assay. Rnsults At the study entry, The positive rate of ANA （ 〉 1 ： 100 ） in LN patients were 100% （ 80/80 ） ,42.5 % （ 34/80 ） LN patients present homogeneous pattern in the sero ,which were significantly more than that in SLE patients （21.79%, 17/78 ） ;While granular pattern in SLE group （73.08% ,57/78 ） was obviously higher than that of LN group （ 53.75 % ,43/80）, P 〈 0.05. In this article, we demon- strated that anti-Hi, anti-Nud, anti-dsDNA antibody were cardinal target antibodies of homogeneous pattern, pearsonx2 were 8.26,23.05,26.39 ,individually ,P 〈 0.05. Through manifestation by chi-spuare test, with regard to LN patients, only preva- lence for anti-dsDNA antibody （55.00% vs. 23.08% ,P = 〈 0.05 ）in patients with LN were significantly higher than that in patients without LN. Conclusions The main fluorescence patterns were homogeneous pattern in sero of SLE accompanied with lupus nephritis. And there were high relationship between ANA homogeneous patterns and anti-dsDNA antibody. As a re- suh, detection of the homogeneous pattern and anti-dsDNA antibody was the effective indicator of diagnosis for LN.