| 荧光定量聚合酶链反应检测去颌下腺大鼠睾丸Bcl 2和Bax mRNA的改变 |
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| 引用本文： | 王晨阳,马百坤,侯林,徐会茹,黄祝,蒋超,黄宇烽,姚兵. 荧光定量聚合酶链反应检测去颌下腺大鼠睾丸Bcl 2和Bax mRNA的改变[J]. 生殖医学杂志, 2008, 17(3): 211-215 |
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| 作者姓名： | 王晨阳 马百坤 侯林 徐会茹 黄祝 蒋超 黄宇烽 姚兵 |
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| 作者单位： | [1]南京军区南京总医院解放军临床检验医学研究所,南京210002 [2]南京师范大学生命科学学院,南京210097 |
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| 基金项目： | 江苏省自然科学基金 , 解放军医药卫生科研项目 |
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| 摘 要： | 目的观察切除颌下腺对大鼠睾丸Bax和Bcl 2 mRNA表达的影响。方法切除大鼠颌下腺,分别于术后14、28和42 d处死大鼠,提取睾丸总RNA,反转录,设计特异性引物和Taqman探针,荧光定量聚合酶链反应(PCR)检测Bax和Bcl 2 mRNA的改变。结果随着颌下腺切除时间的延长,实验组大鼠睾丸Bax和Bcl 2 mRNA的比值显著升高,与对照组比较,有显著性差异(P<0.01)。结论颌下腺切除大鼠睾丸Bax和Bcl 2 mRNA比值明显升高,提示颌下腺切除所致的大鼠睾丸生精细胞的凋亡可能是由Bax和Bcl 2介导的线粒体途径进行,但Fas/FasL是否参与该凋亡过程还有待进一步研究。
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| 关 键 词： | 荧光定量聚合酶链反应 颌下腺 睾丸 Bcl2 Bax 大鼠 |
| 文章编号： | 1004-3845(2008)03-0211-05 |
| 修稿时间： | 2007-05-23 |
Detection of Bcl 2 and Bax mRNA expression with fluorogenic quantitative PCR in rat testis after submandibular glands removed |
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| WANG Chen-yang,,MA Bai-kun,HOU Lin,XU Hui-ru,HUANG Zhu,JIANG Chao,HUANG Yu-feng,YAO Bing. Detection of Bcl 2 and Bax mRNA expression with fluorogenic quantitative PCR in rat testis after submandibular glands removed[J]. Journal of Reproductive Medicine, 2008, 17(3): 211-215 |
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| Authors: | WANG Chen-yang MA Bai-kun HOU Lin XU Hui-ru HUANG Zhu JIANG Chao HUANG Yu-feng YAO Bing |
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| Affiliation: | WANG Chen-yang1,2,MA Bai-kun1,HOU Lin1,XU Hui-ru1,HUANG Zhu1,JIANG Chao1,HUANG Yu-feng1,YAO Bing11.Institute of Clinical Laboratory Medicine,NanjingGeneral Hospital of Nanjing Military Comm,,Nanjing 2100022.College of Life Science,Nanjing Normal University,Nanjing 210097 |
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| Abstract: | Objective: To detect the changes of Bax and Bcl 2 mRNA expression in rat testis after the removal of submandibular glands.Methods: On day 14,28 and 42 after operation(bilateral submandibular glands removed or sham-operated),the levels of Bax and Bcl 2 mRNA in rat testis were examined by using fluorogenic quantitative RT-PCR.Results: Fluorogenic quantitative PCR showed that there was a significant increase in the ratio of Bax/Bcl 2 mRNA in rat testis on day 14,28 and 42 after submanibular glands removed(P<0.... |
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| Keywords: | Fluorogenic quantitative PCR Submandibular gland Testis Bcl 2 Bax Rat |
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