复方活血汤对免疫诱导再生障碍性贫血小鼠骨髓微环境的作用研究

目的 :探讨临床上治疗再生障碍性贫血 (再障 )加用活血化瘀药物提高疗效的机制。方法 :建立免疫介导的再障小鼠模型 ,胃饲 1 0 0 %复方活血汤注射液每次 0 2ml,每天 2次 ,第 1 0天观察骨髓组织学、体外成纤维细胞集落形成单位 (CFU -F)、培养基质细胞层的粘附能力 ,骨髓氧分压 (PO2 )。结果 :复方活血汤组小鼠白细胞计数 ,骨髓有核细胞计数 ,骨髓造血组织容量、CFU -F计数 ,均较再障组有明显升高 (P <0 0 1 ) ;且基质细胞粘附功能、骨髓PO2 已恢复至正常。结论 :复方活血汤可促进再障小鼠骨髓微环境的修复及供氧 ,从而促进骨髓造血。

Effects of Composite Blood-Activating Decoction on Bone Marrow Microenvironment in Mice of Immune-Induced Aplastic Anemia

Objective: To explore the mechanism of elevating efficacy for aplastic anemia (AA) by using blood activating and stasis eliminating drugs. Methods: Immune induced aplastic anemia model was established. Each mouse was gastrogavaged by 0.2 ml 100% composite blood activating decoction (CBAD) twice a day. On the 10th day, the bone marrow histology, CFU F, adhesive function of the cultured stromal cell layer, bone marrow PO 2 were observed. Results: In CBAD group, the WBC count, bone marrow karyocytes, bone marrow hematopoietic tissue volume, CFU F count were significantly higher than those in AA group (P<0 01). Moreover, the adhesive function of stromal cells and bone marrow PO 2 recovered to normal level. Conclusion: The commonly used composite blood activating decoction could promote the recovery and oxygen supply of bone marrow microenvironment in AA mice, and improved the bone marrow hematopoiesis.