Bmi-1和p16基因在膀胱移行细胞癌中的表达及临床意义

目的 检测Bmi-1和p16基因在膀胱移行细胞癌组织中的表达,并探讨其临床意义.方法 采用实时荧光反转录定量聚合酶链反应方法检测61例膀胱移行细胞癌组织和12例正常膀胱组织中Bmi-1和p16基因的表达情况.结果 膀胱移行细胞癌组织中Bmi-1表达量显著高于正常膀胱组织（0.242±0.129比0.031±0.011）,而p16表达量显著低于正常膀胱组织（0.059±0.021比0.165±0.029）,差异均有统计学意义（P＜0.05）.Bmi-1和p16基因的表达量与病理分级、临床分期、肿瘤复发有相关性（P＜ 0.05或＜0.01）,而与年龄、性别无相关性（P＞0.05）.在膀胱移行细胞癌组织中p16基因的表达量与Bmi-1基因的表达量呈负相关（rs=-0.714,P＜0.05）.结论 Bmi-1基因的高表达和p16基因的低表达可能参与了膀胱移行细胞癌的发生和发展过程,Bmi-1可能通过某种机制下调p16的表达,从而促进膀胱移行细胞癌的发生和发展.

Expression of Bmi-1 and p16 gene in transitional cell carcinoma of bladder

Objective To detect the expression of Bmi-1 and p16 gene in transitional cell carcinoma of bladder（TCC） tissue and explore its clinical significance. Methods The expression of Bmi-1 and p16 gene were detected by real-time quantitative polymerase chain reaction in 61 cases of TCC tissue and 12 cases of normal bladder tissue. Results The expression of Bmi-1 gene in TCC tissue was significantly higher than that in normal bladder tissue （0.242 ±0. 129 vs. 0.031 ±0.011 ）, and the expression of p 16 gene was significantly lower than that in normal bladder tissue （0.059 ±0.021 vs. 0.165 ± 0.029）,there was significant difference （P 〈 0.05）. The expression of Bmi-1 and p16 gene were highly correlated withpathological grades, clinical stages and tumor recurrence （P 〈 0.05 or 〈 0.01 ）. But there were not correlated with age and gender （P 〉 0.05 ）.There was a negative correlation between the expression of Bmi-1 gene and p16 gene in TCC tissue（rs = -0.714,P〈 0.05）. Conclusions Bmi-1 gene high expression and p16 gene low expression may be involved in the occurrence and development process of TCC. Bmi-1 may decrease the expression of p 16 gene in some ways, and then lead to the occurrence and development of TCC.