维生素A缺乏对初生鼠FUT8表达与心脏发育关系的影响

目的 本研究通过研究维生素A缺乏(vitamin A deficiency, VAD)对初生鼠FUT8(α-1,6fucosyltransferase, α-1,6FUCT)的表达,探讨VAD对初生鼠心脏发育的关系及其在糖分子水平上可能的机制。方法 通过美国营养学会的改良配方建立VAD大鼠模型。VAD饲料喂养母鼠及所产仔鼠为实验组(VAD组),正常饲料喂养母鼠及所产仔鼠为对照组,在初生鼠中进行比较两组FUT8表达与心脏发育。通过高效液相色谱检测维生素A含量并比较两组产仔率,利用HE染色观察两组心脏发育,采用反转录聚合酶链反应和小扁豆凝集素点印迹的方法检测两组初生鼠FUT8的表达。结果 对照组母鼠产仔率高于VAD组(P<0.001);RT-PCR及LCA凝集素点印迹示VAD组子鼠FUT8的表达量在转录水平和蛋白水平均高于对照组;HE染色发现VAD组子鼠心血管内皮细胞形态变得不规则、分散,而且VAD组心肌细胞胞质变得疏松。结论 维生素A缺乏组会引起FUT8表达的升高,影响心肌细胞形态的改变,这说明VAD与心脏发育有关,维生素A可能促进心脏发育。

Effects of Vitamin A Deficiency on Neonatal Rat FUT8 Eexpression and the Development of the Heart

Objective In this article, we studied the expression of FUT8 in neonatal rat to investigate the influence of Vitamin A deficiency (VAD) in the development of the neonatal rat's cardiovascular system and the potential mechanism. Methods We established VAD rat model by treating with VAD feed using improved AIN-76^TM formula. Rats and its offspring treated with VAD feed were set as the experimental group (VAD group), The normal feed rats and its offspring were set as the control group. The fertility rate between two groups was compared. And the vitamin A in the VAD diet was detected using HPLC. The cardiovascular morphological of the two groups was observed by HE staining. RT-PCT and Lectin blot were employed to detect the expression of FUT8 in the two groups. Results The control group had a higher fertility rate.The expression of FUT8 detected by RT-PCT and Lectin blot was significantly increased in VAD group. The morphological of the offspring rats' cardiovascular endothelial cells in the VAD group, as showing in the HE staining, became irregular, scattered and loose. Conclusion VAD could induce the over-expression of FUT8 and affect the morphological of cardiac cell, which suggestes that VAD has an important role in the heart development and Vitamin A might promote the development of heart.