抗生素治疗大鼠烧伤绿脓杆菌菌血症时释放内毒素的实验研究

目的 观察不同种类抗生素治疗烧伤绿脓杆菌(PA103)菌血症时诱导细菌释放内毒素的情况。方法利用内毒素微量检测技术，检测抗生素治疗烧伤菌血症大鼠血中内毒素水平及血中细菌菌量。结果 应用敏感抗生素治疗烧伤菌血症均能有效杀灭细菌，但同时能不同程度诱导细菌释放内毒素，伊米配能诱导释放内毒素较少，头孢哌酮次之，头孢他啶和头孢氨噻肟较多。结论 不同种类抗生素具有不同程度的诱导绿脓杆菌释放内毒素的作用，释放的量与其杀菌能力无相关。

An experimental study of antibiotic-induced endotoxin release in Pseudomonas aeruginosa bacteremia inburned rats

OBJECTIVE: To assess the intensity of endotoxin release in Pseudomonas aeruginosa challenged burned rats after the administration of various antibiotics. METHODS: Rats were inflicted with 30% TBSA burn, and they were challenged with Pseudomonas aeruginosa (PA103). After the administration of different beta lactam antibiotics, serum endotoxin level and blood bacterial count were determined. RESULTS: All the antibiotics used were effective in eradicating the bacteria with concomitant release of endotoxin in varying amounts. It was found that Imipenem released the least amount of endotoxin, followed in order by Cefoperazone, Ceftazidime, and Cefotxime. CONCLUSIONS: Different antibiotics possessed varying capacity of inducing endotoxin release from PA, and the amount released did not bear any relation with the bactericidal capability of the antibiotics.