脑外伤后脑线粒体功能和结构改变及神经节苷脂治疗作用

目的从牛脑组织中提取神经节苷脂ＧＭ１，研究其对脑外伤后脑细胞呼吸功能和结构保护作用。方法把ＳＤ大鼠分成正常组，生理盐水治疗的损伤对照组及ＧＭ１治疗的损伤治疗组。结果发现对照组伤后８小时和１６小时Ⅲ态呼吸耗氧速率（Ｒ３），呼吸控制率（ＲＣＲ），磷／氧比（Ｐ／Ｏ）及氧化磷酸化效率（ＯＰＲ）有明显降低，治疗组则明显增加，且与正常组相近。对照组皮层神经元细胞和线粒体超微结构有明显损害，治疗组损害明显减轻。结论其可能机制与ＧＭ１保护膜脂和膜酶活性，维持膜内外离子平衡，减轻水肿及减少自由基形成等有关

Protective effects of GM 1 ganglioside on cerebral cellular respiratory function and ultrastructure after traumatic brain injury

Objective To assess the protective effects of GM 1 ganglioside on cerebral cellular respiratory function, and to study the ultrastructure changement after traumatic brain injury in rat model. Methods Sprague Dawley rats were divided into three groups: normal group, 0 9% sodium chloride solution treated control group and GM 1 treated group. Results The results showed R 3(oxygen uptake rate in state 3 respiration), RCR(respiratory contral ratio) and P/O(the molar ratio inorgenic phosphate esterified and oxygen reduced), OPR(oxidative phosphoxylation rate) decreased significantly in contral group on 8th and 16th hour after injury, while increaed dominantly in GM 1 treated group, which were similar to those in normal group. Cortical neurons and their mitochondrial ultrastructure of control group were severely damaged, while the pathological changes in GM 1 treated group were much milder. Conclusion The possible mechanism is that GM 1 keeps normality of the plasma membrane lipids, and membrane enzyme activity, and reduces ionic imbalance, edema and free radical formation.