| 胶质瘤细胞的ERCC—2基因表达和氯乙基亚硝脲耐药 |
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| 引用本文： | 陈忠平 Mohr.,G. 胶质瘤细胞的ERCC—2基因表达和氯乙基亚硝脲耐药[J]. 中华神经外科杂志, 1999, 15(6): 365-367 |
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| 作者姓名： | 陈忠平 Mohr. G |
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| 作者单位： | 苏州医学院附二院神经外科!２１５００４,SirMortimerBDavis-JewishGeneralHospital,NcGillUniversityMontreal,Canada,SirMortimerBDavis-JewishGeneralHospital,NcGillUniversityMontreal,Canada,苏州医学院附二院神经外科!２１５００４ |
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| 摘 要： | 目的探讨核苷酸切除修复交叉互补（ＥＲＣＣ）基因表达与肿瘤细胞对氯乙基亚硝脲（ＣＥＮＵ）耐药的关系。方法采用我们已建立的逆转录．聚合酶连锁反应（ＲＴ－ＰＣＲ）定量分析方法,对９株人胶质瘤细胞的ＥＲＣＣ－２基因表达进行测定,并与ＣＥＮＵ细胞毒性试验进行相关分析。结果ＥＲＣＣ－２基因表达与二氯乙基亚硝脲（ＢＣＮＵ）及二氯乙基肉芥子亚硝脲（ＳａｒＣＮＵ）耐药性之间有显著的正相关（ｒ＝０．６７２,Ｐ＝０．０４７和ｒ＝０．７２６,Ｐ＝０．０２８）。结论本研究提示ＥＲＣＣ－２基因表达在胶质瘤对亚硝脲类抗癌药耐药中起重要作用。
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| 关 键 词： | 核苷酸切除修复交叉互补 胶质瘤 化疗 耐药 逆转录－聚合酶连锁反应 |
ERCC-2 Gene expression and chloroethylnitrosourea in 9 human glioma cell lines |
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| CHEN Zhong-ping,Gerard Mohr,Lawrence Panasci et al. ERCC-2 Gene expression and chloroethylnitrosourea in 9 human glioma cell lines[J]. Chinese Journal of Neurosurgery, 1999, 15(6): 365-367 |
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| Authors: | CHEN Zhong-ping Gerard Mohr Lawrence Panasci et al |
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| Affiliation: | Department of Neurosurery Xecond Affiliated Hospital of SuzhouMedical College Suzhou 215004 |
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| Abstract: | Objective To investigate concordance between nucleotide excision repair(NER)gene expressionand chloroethylnitrosourea (CENU) resistance in human tumor cell lines. Methods ERCC-2 gene expressions in9 human glioma cell be were evaluated by using an established quantitative revese-transcription polymerase chain reaction (RT-PCR). Correlation analysis between the results and CENU cytotoxicities was done. Results There wasa significant correlation between ERCC-2 gene expression and BCNU or SarNU cytotoxicity (r=0.672, P=0.047and r=0.726, P=0.028 respectively). Conclusion Our results suggest that ERCC-2 gene expression may playan important role in nitrosoureas drug resistance in human gliomas |
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| Keywords: | ERCC-2 Glioma Chemotherapy Drug resistance RT-PCR |
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