| 一种新酶放大系统及其应用 |
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| 引用本文： | 王Min 戴信雄.一种新酶放大系统及其应用[J].中国药科大学学报,1995,26(1):55+53-55. |
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| 作者姓名： | 王Min 戴信雄 |
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| 作者单位： | 中国药科大学生物化学教研室，美国肯塔基大学药学院 |
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| 摘 要： | 建立了一种能广泛用于酶标免疫吸附分析的新酶放大系统。该系统采用Ｌ－乳酸脱氢酶、乳酸氧化酶、辣根过氧化物酶、ＮＡＤＨ和３，３＇，５，５＇－四甲基联苯胺组成一个循环体系，用以放大碱性磷酸酶产生的信号。与其它酶放大系统相比，具有灵敏度高和成本低的特点。
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| 关 键 词： | 酶放大系统 酶免疫分析 ELISA |
A New Enzyme Amplification System and Its Application |
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| Wang Min,Tai Hsin-Hsiung.A New Enzyme Amplification System and Its Application[J].Journal of China Pharmaceutical University,1995,26(1):55+53-55. |
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| Authors: | Wang Min Tai Hsin-Hsiung |
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| Institution: | Wang Min,Tai Hsin-Hsiung Department of Biochemistry,China Pharmaceutical University,Nanjing 210009,College of Pharmacy,University of Kentucky,Lexington,KY 40536-082 USA) |
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| Abstract: | A new enzyme amplification system which can be widely used in ELISA was developed.In this system,a complex of L-lactic dehydrogenase,lactate oxidase,horseradish peroxidase,NADH and 3,3',5,5'-tetramethylbenzidine were used to compose a cycling system to increase the signal for the assay of alkaline phosphatase.Comparison with other methods showed that it gave high sensitivity and low background. |
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| Keywords: | enzyme amplification enzyme immunoassay ELISA |
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