| 非小细胞肺癌端粒酶活性的相对定量检测 |
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| 关 键 词： | 肺肿瘤 癌 非小细胞性 端粒酶 |
Detection of telomerase activity level in human non-small-cell lung cancer] |
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| H Z Yang,C P Hu,X L Su. Detection of telomerase activity level in human non-small-cell lung cancer][J]. Bulletin of Hunan Medical University, 2001, 26(6): 549-550 |
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| Authors: | H Z Yang C P Hu X L Su |
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| Affiliation: | Department of Respiratory Disease, Xiangya Hospital, Central Suth University, Changsha 410008, China. |
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| Abstract: | OBJECTIVE: To investigate the telomerase activity in human non-small-cell lung cancer (NSCLC) and to investigate the possibility of telomerase as a tumor biological marker. METHODS: The semi-quantitative assay and expression of PCR-based telomeric repeats amplification Protocol ELISA (TRAP-PCR-ELISA) and PAGE (TRAP-PCR-PAGE) were detected in 15 NSCLC tissues and 15 tumor-adjacent tissues, 3 normal lung tissues. RESULTS: No telomerase activity in 15 tumor-adjacent tissues and 3 normal lung tissues was detected, However, of 15 NSCLC tissues, 13 were positive for telomerase activity with a 86.7% positive rate, telomerase activity was significantly higher in NSCLC than in Tumor-adjacent tissues and normal lung tissues (P < 0.001). CONCLUSION: The results suggest that telomerase is a tumor specific gene marker. |
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