葛根素对铝致痴呆模型小鼠学习记忆

目的 探讨葛根素(Pur)对铝中毒所致的痴呆模型小鼠记忆能力的影响及机制.方法 给小鼠连续注射AlCl3建立AD模型,用Morris水迷宫法测试小鼠的空间记忆能力,用黄嘌呤氧化酶法测定海马SOD活力,以硫代巴比妥酸法测定海马MDA含量.结果 与正常对照组相比,模型组海马SOD活力(32.11±18.53)U/mag prot]下降(P<0.01)、blDA含量(3.94±0.61)nmol/mg prot]上升(P<0.01),同时伴有水迷宫成绩的损害.与模型组比较,Pur中、高剂量组海马SOD活力(58.63±17.92)U/mg prot,(61.29±19.47)U/mg prot]上升(P<0.01)、MDA含量(2.93±0.31)nmol/mg prot,(2.89±0.45)nmol/mg prot]下降(P<0.01),Pur低剂量组海马SOD活力(49.74±16.37)U/mg prot]上升(P<0.05),Pur中、高剂量组对水迷宫成绩的改善作用优于低剂量组.结论 Pur能改善铝中毒所致的痴呆模型小鼠记忆能力,其作用机制可能与其抗氧化作用有关.

The effects of puerarin on learning memory ability and level of hippocampal SOD and MDA in mice model of Alzheimer's disease induced by AlCl3

Objective To study the effect of puerarin (Pur) on memory ability of AD mice induced by alu-minum poisoned and the involved mechanism. Methods AlCl3 was injected several times to establish Alzheimer's disease model mice. Spatial memory ability were tested by Morris water maze,Activity of SOD and content of MDA in the hippocampus were measured by Xanthine oxidase method and Thiobarbituric acid method respectively. Re-sults As compared with the normal control groups, the activity of hippocampal SOD was declined 32.11±18.53 )U/mg prot, P<0.01],but the content of MDA was increased the results associated with lower Performance in Water Labyrinth. (3.94±0.61)nmol/mg prot, P<0.01] in model groups. Compared with the model groups,the groups treated with the pur could markedly improve learning memory ability. The activity of SOD was rised (58.63±17.92 ) U/mg prot,(61.29±19.47) U/mg prot, P<0.01], the level of M DA was decreased (2.93 ±0.31) nmol/mg prot, (2.89±0.45)nmol/mg prot, P<0.01] in high and Medium dose of Pur. The activity of SOD was also rised (49.74±16.37) U/mg prot, P<0.05]in low dose groups. The Performance of high dose was better than the low dose in water maze. Conclusions The Pur can significantly improve learning and memory ability in aluminum lesioned mice. and the mechanism involved might closely correlate with its roles of antioxida-tion.