碱性成纤维细胞生长因子促血管内皮细胞增生的研究

目的 探讨碱性成纤维细胞生长因子(bFGF)对人血管内皮细胞(EC)增生的影响及其机制，明确bFGF对烟曲霉素衍生物(TNP-470)及地塞米松(Dex)作用的影响。方法 培养人脐静脉内皮细胞(HUVEC)，采用MTT法测定EC生长活性，免疫组织化学方法检测EC中核因子(NF—KB)和ki-67的表达。结果 bFGF有显著促进EC增生的作用，可使核内NF-κB和ki-67表达增强ITNP-470及Dex可抑制EC增生，使核内NF—κB和ki-67表达减少；bFGF逆转二者的抑制效应。结论 bFGF能促进EC增生，其机制可能是通过激活NF—κB，使细胞由静止期进入增殖期，促进DNA合成，细胞分裂增殖。TNP-470及Dex可抑制NF—κB活化，bFGF可逆转二者的抑制效应。

ENDOTHELIAL CELL PROLIFERATION STIMULATED BY BASIC FIBROBLAST GROWTH FACTOR

OBJECTIVE: To determine the influence of basic fibroblast growth factor (bFGF) on endothelial cell (EC) proliferation in vitro and its possible mechanisms, and to examine the effect of both TNP-470 and dexamethasone (Dex) on the EC proliferation induced by bFGF. METHODS: Human umbilical vein endothelial cells were cultured and the proliferation of EC was quantified by a colorimetric assay using MTT reagent. The expression of nuclear factor-kappa B (NF-kappa B) and ki-67 was detected with SABC immunohistochemical method. RESULTS: bFGF stimulated the EC proliferation and enhanced the expression of NF-kappa B and ki-67 in nucleus; TNP-470 and Dex suppressed EC proliferation induced by bFGF, and reduced the expression of NF-kappa B and ki-67 in nucleus. CONCLUSION: The above results indicate that the possible mechanisms of EC proliferation stimulated by bFGF come from that bFGF can activate NF-kappa B to promote the synthesis of DNA and EC mitosis. TNP-470 and Dex inhibited EC proliferation stimulated by bFGF by inhibiting NF-kappa B.