| 胰岛素可磁化固相放射免疫分析 |
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| 引用本文： | 李建华,刘慎沛,崔武任,叶维新,张建华,金之欣. 胰岛素可磁化固相放射免疫分析[J]. 华中科技大学学报(医学版), 1991, 0(1) |
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| 作者姓名： | 李建华 刘慎沛 崔武任 叶维新 张建华 金之欣 |
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| 作者单位： | 同济医科大学实验医学研究中心核医学研究室(李建华，刘慎沛，崔武任，叶维新)，同济医科大学附属同济医院内科内分泌室(张建华)，同济医科大学附属同济医院内科内分泌室(金之欣) |
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| 摘 要： | 用胰岛素(INS)免疫豚鼠制得INS抗血清,通过氯胺T法碘化标记INS制备~(125)I-INS,结合本室研制的可磁化固相二抗分离试剂(DAMP),建立了血清INS可磁化固相放射免疫分析法(RIA)。本法灵敏度达0.1μU,回收率>95％,批内变异(CV)<2.3％,批间CV<7.4％。并具有不需离心,操作简便,分离迅速的特点。经临床初步应用,获得满意的结果。
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| 关 键 词： | 胰岛素 放射免疫测定 磁力学 |
Radioimmunoassay for Insulin in Serum with Magnetic Separation of the Solid-phase |
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| Li Jianhua,Liu Shenpei,Cui Wuren,Ye Weixin Nuclear Medicine Research Department,Research Center of Experimental Medicine,Tong/i Medical University,WuhanZhang Jianhua,Jin Zhixin. Radioimmunoassay for Insulin in Serum with Magnetic Separation of the Solid-phase[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 1991, 0(1) |
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| Authors: | Li Jianhua Liu Shenpei Cui Wuren Ye Weixin Nuclear Medicine Research Department Research Center of Experimental Medicine Tong/i Medical University WuhanZhang Jianhua Jin Zhixin |
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| Affiliation: | Li Jianhua,Liu Shenpei,Cui Wuren,Ye Weixin Nuclear Medicine Research Department,Research Center of Experimental Medicine,Tong/i Medical University,WuhanZhang Jianhua,Jin ZhixinDepartment of Internal Medicine,Tongji Hospital,Tongji Medical University,Wuhan |
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| Abstract: | |
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| Keywords: | insulin radioimmunoassay magnetics |
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