| 糖基化终产物对3T3-L1脂肪细胞胰岛素敏感性及SAA3基因表达的影响 |
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| 引用本文： | 叶夏云,薛耀明,沙建平,何飞英,王玲.糖基化终产物对3T3-L1脂肪细胞胰岛素敏感性及SAA3基因表达的影响[J].中华糖尿病杂志,2009,17(8):594-596. |
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| 作者姓名： | 叶夏云 薛耀明 沙建平 何飞英 王玲 |
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| 作者单位： | 南方医科大学附属南方医院内分泌代谢科,广州,510515 |
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| 摘 要： | 目的探讨糖基化终产物（AGE）对3T3-L1脂肪细胞胰岛素敏感性及SAA3基因表达的影响。方法以2-DG摄入法观察葡萄糖的摄取率，用RT-PCR检测脂肪因子SAA3mRNA的表达。结果AGE显著减少3T3-L1脂肪细胞在胰岛素刺激下的葡萄糖摄取，呈剂量和时间依赖效应；AGE显著增加脂肪细胞SAA3mRNA的表达；呈剂量依赖方式。结论AGE能降低3T3-L1脂肪细胞对葡萄糖的摄取，增加3T3-L1脂肪细胞对淀粉样蛋白的表达。
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| 关 键 词： | 糖基化终产物 3T3-L1脂肪细胞 葡萄糖摄取 血清淀粉样蛋白A |
Effect of AGE on insulin sensitivity and expression of SAA mRNA in 3T3-L1 adipocytes |
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| Institution: | YE Xia-yun, XUE Yao-ming, SHA Jian-ping, et al.( Department of Endocrinology, Nan fang Hospital, Guangzhou 510515, China) |
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| Abstract: | Objective To observe the effect of AGE on insulin sensitivity and expression of SAA3 mRNA in 3T3-L1 adipocytes. Methods The glucose uptake rate of the cell was assayed by the uptake of 2-DG. RT-PCR was performed to quantitate the expression of SAA3 mRNA. Results AGE attenuated insulin-stimulated glucose uptake in 3T3-L1 adipocytes in a dose- and time-dependent manners, and expression of SAA mRNA was increased in a dose-dependent manner in 3T3-L1 adipocytes incubated with AGE. Conclusions AGE can inhibit glucose uptake and increase expression of SAA3 mRNA in 3T3-L1 adipocytes. |
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| Keywords: | Advanced glycation end products 3T3-LI adipocytes Glucose uptake Serum amyloid A |
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