硫酸软骨素的提取与精制

目的：探讨以猪喉、气管软骨和鼻软骨为原料制备高纯度的硫酸软骨素的方法。方法：稀碱浓盐提取猪喉、气管软骨和鼻软骨组织，选择相对分子质量为10000的切向流超滤膜进行精制的方法制备。测终产品硫含量，根据硫元素在硫酸软骨素分子中的比例推算Chs含量，用元素分析仪及凯氏定氮法测氮含量。结果：超滤后，以猪喉气管软骨和鼻软骨为原料制得的硫酸软骨素含量分别增加16．83％和11．75％，含量均达98％以上；用2种方法测氮含量分别减少0．4373％，0．4820%和0．2665%，0．5156％，均小于3．8%。结论：该方法简便、可靠，产品质量稳定。

Extraction and refinement of chondroitin sulfate

Aim: To investigate the method of producing high and pure chondroitin sulfate using larynxes and tracheae cartilage,and nose cartilage of pigs as material. Methods: Chondroitin sulfate was extracted by low concentrative base and high concentrative salt, and then refined by tangent ultrafilter method selecting ultrafilter membrane of molecular weighted 10 000. Results: The contents of chondroitin sulfate from larynxes and tracheae cartilage,and nose cartilage were improved by 16.83%, 11.75%, respectively, and were more than 98%. The contents of nitrogen reduced by 0.437 3%, 0.482 0% and 0.266 5%, 0.515 6% by two methods and were less than 3.8%. Conclusion: This method is simple, liable and the quality of the product is stable.