不同菌相白念珠菌对巨噬细胞凋亡的影响

目的：观察不同菌相白念珠菌诱导巨噬细胞凋亡情况,为疾病的预防和治疗提供思路.方法：用佛波醇酯刺激THP-1细胞分化为巨噬细胞,用酵母相、菌丝相及用胃蛋白酶抑制剂（pepstatin,PEP）预处理的白念珠菌分别作用巨噬细胞1、2、4、8 h;另设空白对照组,AnnexinV-FITC染色后流式细胞仪检测细胞的凋亡率.结果：酵母相、菌丝相、PEP作用组的白念珠菌分别感染巨噬细胞1、2、4、8 h的细胞凋亡率均较空白对照组升高（P〈0.05~P〈0.01）;各作用组均随时间的延长凋亡率不断升高（P〈0.01）;酵母相组和菌丝相白念珠菌组不同时间细胞凋亡率均较PEP作用组明显增加（P〈0.01）,并且菌丝相组细胞凋亡率明显高于酵母相组（P〈0.01）.结论：白念珠菌可诱导巨噬细胞凋亡,其作用与其菌相及其分泌型天冬氨酸蛋白酶有关.

The effect of different flora Candida albicans on the apoptosis of macrophage

Objective： To investigate the apoptosis of macrophage induced by different flora Candida albicans, and provide a new insight for the prevention and treatment of disease. Methods：The THP-1 cells were induced to differentiate into macrophage by phorbol myristate acetate. The macrophage was infected by yeast form, hyphal form of Candida albicans and Candida albicans pre-treated with prepstatin（PEP） for 1 hour, 2,4 and 8 hours, the blank control group was also established. The apoptosis rate of macrophage was detected by flow cytometer. Results：Compared with the blank control group, the apoptosis rates of macrophage infected by the yeast form, hyphal form of Candida albicans and Candida albicans pre-treated by PEP for 1 hour,2,4 and 8 hours significantly increased（ P 〈 0.05 to P 〈 0. Ol ）, the apoptosis rate of macrophage increased with the extension of infection time （ P 〈 0.01 ）. Compared with macrophage infected by Candida albicans pre-treated by PEP,the apoptosis rates of macrophage infected by the yeast form and hyphal form of Candida albicans significantly increased in different time-point（ P 〈 0.01 ）. The apoptosis rate of macrophage infected by hyphal form of Candida albicans was significantly higher than that in macrophage infected by yeast form（ P 〈 0.01 ）. Conclusions：Candida albicans can induce the apoptosis of macrophage, the effect of which is related to the form of Candida albicans and secreted aspartic proteinase.