百草枯中毒大鼠胃肠损伤的实验研究

目的:观察百草枯(PQ)中毒大鼠胃肠损伤的病理变化及胃肠组织中肿瘤坏死因子-(TNF-)、白介素-1(IL-1)、超氧化物歧化酶(SOD)及丙二醛(MDA)的表达变化,探讨炎症反应及氧自由基在PQ所致胃肠损伤中的作用。方法:40只SD大鼠,随机分为对照组和中毒组。其中对照组10只用0.9%氯化钠注射液一次性灌胃;中毒组30只给予0.9%氯化钠注射液稀释PQ,按180 mg/kg一次性灌胃,分别于6、24、72 h后处死10只,留取胃肠标本,观察胃肠大体,HE染色的病理改变,并采用免疫组织化学SP法检测胃肠组织中MDA、SOD、TNF-和IL-1的表达,ELISA法检测胃肠组织中TNF-和IL-1的表达。结果:PQ中毒大鼠胃肠有明显病理损伤;中毒组大鼠胃肠组织中TNF-、IL-1及MDA的表达均明显高于对照组(P0.01);中毒组SOD表达明显低于对照组(P0.01)。结论:PQ中毒大鼠胃肠有明显病理损伤,炎症反应及氧自由基在PQ所致胃肠损伤中可能起重要的作用。

The experimental research of gastrointestinal injury induced by paraquat in rats

Objective： To investigate the pathological change and expression of malondialdehyde （MDA）, superoxide dismutase （SOD）, tumor necrosis factor-α （TNF-α） and interleukin-1β （IL-1β） in gastrointestinal tissue of rats with acute paraqnat （PQ） poisoning. Methods：Forty SD rats were randomly divided into control group and poisoned group（ 10 rats and 30 rats, respectively）. The control group and poisoned group were disposably perfused with normal saline and 180 mg/kg of the diluent of normal saline and PQ, respectively. The gastrointestinal tissue of control group（ 10 rats） and 10 rats of poisoned group at 6,24 and 72 h after treatment were harvested. Gastrointestinal gross morphology was observed. The pathological change, the expression of MDA, SOD, TNF-α and IL-1β in gastrointestinal tissue were detected by immunohistochemistry（ IHC）, and the expression of TNF-α and IL-1β in gastrointestinal tissue were detected by ELISA. Results ：There were obviously pathological injury in gastrointestinal tissue of rats with acute PQ poisoning. The expressions of MDA, TNF-α and IL-1β in poisoned group were significantly higher than those in control group （ P 〈 0. 01 ）, the expression of SOD in poisoned group was significantly lower than that in control group （P 〈 0.01 ）. Conclusions：There are obviously pathological injury in gastrointestinal tissue of rats with acute PQ poisoning. The inflammatory response and oxygen free radicals may play an important role in gastrointestinal injury of poisoned rats.