不同血液标本及存放时间对神经元特异性烯醇化酶测定的影响

目的：探讨室温下不同血液标本及存放时间对神经元特异性烯醇化酶（NSE）检测结果的影响.方法：分别用分离胶/促凝剂管（分离胶管组）和肝素抗凝管（肝素管组）采集30名健康体检人员的血标本,在1 h内离心并测定标本中NSE水平,然后分别测定室温（25.0±0.5）℃放置2、3、4 h的NSE水平.结果：分离胶管组血标本存放不同时间的测定结果与1 h内的NSE测定结果差异无统计学意义（P〉0.05）;但肝素管组血标本存放不同时间的NSE测定结果差异有统计学意义（P〈0.01）;肝素管组血标本存放1~4 h NSE测定结果均明显高于分离胶管组（P〈0.01）.结论：应用全自动电化学发光免疫分析系统检测室温放置4 h内的分离胶/促凝剂管血标本,重复性较好,结果可靠,可为临床样本检测、复核,提高分析前质量控制提供科学依据.

The effect of blood sample type and storage time on the assay of neuron-specific enolase

Objective：To investigate the effects of blood sample type and storage time on the assay of neuron-specific enolase（NSE） at room temperature. Methods： Blood samples of 30 healthy people were stored in tube containing gel/coagulant and heparin, respectively. The NSE levels of all samples were determined within 1 hour, the NSE levels of all samples were determined again when they were placed for 2 hours,3 hours and 4 hours at room temperature （ 25.0 ± 0.5 ）℃. Results ： Compared with the results of sample storing in gel/coagulant within 1 hour, the differences of NSE levels at different storage time had no statistical significance （P 〉 0.05）, the differences of sample storing in heparin had statistical significance （ P 〈 0.01 ）. The NSE level of sample storing in heparin was significantly higher than that in sample storing in gel/coagulant for 1 hour to 4 hours （P 〈 0.01 ）. Conclusions： The resuhs of blood sample storing in gel/coagulant for 4 h were good and reliable, which can provide the scientific basis in detecting, re-checking and analysing sample.