N-乙酰半胱氨酸对早期日本血吸虫病小鼠肝脏虫卵肉芽肿的影响

目的 研究N-乙酰半胱氨酸（NAC）对早期日本血吸虫病小鼠肝脏虫卵肉芽肿的影响。 方法　 将36只健康小鼠随机分为NAC组、正常对照组、感染对照组。NAC组和感染对照组小鼠每只经腹部皮肤感染25±2条日本血吸虫尾蚴,于感染当天起给NAC组小鼠200 mg/kg NAC灌胃,2次/d,共42 d;正常对照组和感染对照组每只小鼠2 ml蒸馏水灌胃,2次/d,共42 d。感染第42天,各组小鼠全部处死后取血清和肝脏。对肝脏进行病理学观察,测定血清及肝组织的生化指标。 结果 NAC组小鼠的肝组织“+、++、+++”3级单个虫卵肉芽肿个数分别为1.80±0.25、1.37±0.23、0.53±0.15, 均显著低于感染对照组（P＜0.05）。NAC组小鼠血清中NO水平、GSH水平分别为0.53±0.17、229.66±9.47,相对感染对照组均有所下降（P＜0.05）,谷胱甘肽过氧化物酶GSH-PX活性为1101.99±140.81, 相对感染对照组有所升高（P＜0.05）;肝组织诱导型一氧化氮合酶（iNOS）活性、NO水平、还原型谷胱甘肽（GSH）水平及GSH-PX活性分别为6.85±0.30、13.44±0.40、358.40±19.15、110.84±10.93,均显著低于感染对照组（P＜0.05）。 结论 N-乙酰半胱氨酸可以减缓日本血吸虫病小鼠肝组织病理变化。

Effect of N-acetylcysteine on the Egg Granuloma in Hepatic Tissue of Mice with Schistosomasis japonica

OBJECTIVE: To study the effect of N-acetylcysteine (NAC) on the egg granuloma in hepatic tissue of mice infected with Schistosoma japonicum. METHODS: 36 mice were randomly divided into normal group, infected group and NAC group, each with 12 mice. The mice in the latter two groups were each infected with 25+/-2 cercariae of S. japonicum through the skin of abdomen. NAC solution was orally given to the mice of NAC group, 200 mg/kg, 2 times/d from the day of infection through to the 42nd day. Mice in the other 2 groups were given 2 ml normal saline daily. The mice were all sacrificed at the end of the 42nd day and their livers were collected for pathologic observation. Area of the egg granuloma was measured with computer image analysis software. Concentration of nitric oxide (NO) and reduced glutathione hormone (GSH), and the activity of glutathione peroxidase (GSH-PX) in serum and hepatic tissue, and the activity of inducible nitric oxide synthase (iNOS) in the hepatic tissue were all detected. RESULTS: Number of the single egg granuloma of "+,++,+++" grade were 1.80+/-0.25, 1.37+/-0.23 and 0.53+/-0.15 respectively in NAC treated group, which were less than those of infected group (3.70+/-0.28, 2.77+/-0.25 and 2.00+/-0.14 respectively) (P<0.05). The serum NO and GSH concentration was 0.53+/-0.17 and 229.66+/-9.47 respectively in NAC group, lower than those of infected group (2.64+/-0.31 and 312.47+/-18.55 respectively) (P<0.05), but its GSH-PX activity was 1101.99+/-140.81, higher than that of infected group (663.66+/-25.59) (P<0.05). The concentration of NO and GSH, and the activity of iNOS and GSH-PX in hepatic tissue of NAC group were 6.85+/-0.30, 13.44+/-0.40, 358.40+/-19.15 and 110.84+/-10.93 respectively, lower than those in infected group (8.26+/-1.69, 28.40+/-0.56, 1132.44+/-52.82 and 226.26+/-16.25 respectively) (P<0.05). CONCLUSION: NAC may have the effect of retarding pathological change of the liver, which may associate with the decrease of NO and GSH in serum and hepatic tissue and iNOS activity in the tissue.