大鼠视网膜缺血再灌注损伤后碱性成纤维细胞生长因子对热休克蛋白70表达的影响

目的 观察大鼠视网膜缺血再灌注损伤后热休克蛋白70(HsP70)的表达以及外源性碱性成纤维细胞生长因子(bFGF)对其表达的影响。 方法 采用升高眼内压的方法，制作实验性视网膜缺血再灌注损伤大鼠模型。将24只wistar大鼠随机分为正常组(3只)和手术组(21只)。其中手术组大鼠左眼为缺血再灌注组，右眼为bFGF治疗组(玻璃体腔内注射bFGF 2肛g)，手术组根据再灌注后不同时间分为。、4、8、12、24、48、72 h组。应用免疫组织化学方法观察大鼠视网膜缺血再灌注损伤后视网膜内层组织中HsP70的表达及玻璃体腔内注射外源性bFGF对其表达的影响。 结果 对照组无阳性细胞表达。缺血再灌注组中，缺血再灌注O h后即可见HsP70的表达(20．8±4．5)个／mm。]，并随时间延长而逐渐增加，至24 h达到高峰(111．2±4．4)个／mm z]，随后阳性细胞递减，72 h时偶见阳性细胞。bFGF治疗组HsP70的表达变化规律与缺血组基本一致，但在8、12、24、48、72 h时均较缺血再灌注组显著增高(P<0.05).

The effects of basic fibroblast growth factor on the expression of heat shock protein 70 in rat′s retina afterischemia/reperfusion injury

Objective To observe the effects of basic fibroblast growth factor (bFGF) on the expression of heat shock protein 70 (HSP70) in ratrs retina after iscbemia/reperfusion injury.Methods The rat model of experimental retinal ischemia/reperfusion injury was made by increasing the intraocular pressure. Tweenty-four Wistar rats were divided into normal (3 rats) and operation group (21 rats) randomly. The latter group was subdivided into group 0 hour, 4, 8, 12, 24, 48 and 72 hours after reperfusion, in which the left eyes of the rats were in the ischemia/reperfusion groups and the right ones were in the treatment groups (bFGF 2 t~g intracameral injection). The expression of HSP70 was observed by strept avidin-biotin complex (SABC) immunohistochemistry. Results No HSP70 positive cells were found in normal group; a few of HSP70 positive cells were found 0 hour after reperfusion 20.8±4. 5) cells/mm2], and increased gradually until reached the peak 24 hours later (111.2±4.4) cells/mm2] and then decreased gradually. Few HSP70 positive cells were found 72 hours after reperfusion. The amount of HSP70 positive cells increased in treatment group at all time courses, and the peak time was earlier and longer than that in ischemia group. HSP70 positive cells distributed extensively in retinal ganglion cell layer and inner nucleous layer. The difference of the amount of HSP70 positive cells between the two groups was significant (P<0.05) 8, 12, 24, 48 and 72 hours after reperfusion.Conclusion bFGF can enhance the expression of HSP70 in rat’s retina after retinal ischemia/reperfusion injury.(Chin J Ocul Fundus Dis，2004，20：37-39)