| 成骨细胞与血管内皮细胞联合培养对成骨细胞功能的影响 |
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| 引用本文： | 张健,胡敏,张文怡.成骨细胞与血管内皮细胞联合培养对成骨细胞功能的影响[J].华西口腔医学杂志,2005,23(4):325-328. |
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| 作者姓名： | 张健 胡敏 张文怡 |
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| 作者单位： | 1.解放军总医院 口腔颌面外科,北京100853;2.锦州医学院附属口腔医院 口腔颌面外科,辽宁 锦州121000 |
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| 基金项目： | 全军“十五”医药卫生科研基金面上课题资助项目(01MA111) |
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| 摘 要： | 目的 探讨不同比例兔成骨细胞(ROB)与血管内皮细胞(RVEC)直接联合共培养后,对成骨细胞功能的影
响。方法 取兔成骨细胞与血管内皮细胞,将两者分为4组分别以1∶0、1∶1、2∶1、4∶1直接联合共培养于培养皿中,
通过对细胞内碱性磷酸酶活性(ALP)测定和培养液中骨钙素(OC)定量测定,观察血管内皮细胞对成骨细胞功能的
影响。结果 成骨细胞与血管内皮细胞直接联合共培养相容性良好,2∶1的细胞比例组ALP活性及OC含量较其
他各组明显增高。结论 在体外直接联合共培养的体系中,血管内皮细胞有明显促进成骨细胞活性的作用。
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| 关 键 词： | 成骨细胞 血管内皮细胞 碱性磷酸酶 骨钙素 |
| 文章编号： | 1000-1182(2005)04-0325-04 |
| 收稿时间： | 2004-11-14 |
| 修稿时间： | 2004年11月14 |
Influence of Vascular Cell Co-cultured with Osteoblast in vitro on Ability of Osteoblast |
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| ZHANG Jian,HU Min,ZHANG Wen-yi.Influence of Vascular Cell Co-cultured with Osteoblast in vitro on Ability of Osteoblast[J].West China Journal of Stomatology,2005,23(4):325-328. |
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| Authors: | ZHANG Jian HU Min ZHANG Wen-yi |
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| Institution: | 1.Dept. of Oral and Maxillofacial Surgery, PLA General Hospital, Beijing100853,China;2. Dept. of Oral and
Maxillofacial Surgery,Hospital ofStomatology, Affiliated to Jinzhou Medical College, Jinzhou121000,Chin |
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| Abstract: | OBJECTIVE; The purpose of this study was to observe the influence on proliferation of rabbit osteoblast (ROB) of rabbit vascular endothelial cell(RVEC) co-cultured with rabbit osteoblast (ROB). METHODS: Rabbit osteoblasts and rabbit vascular endothelial cells in ratio of 1:0, 1:1, 2:1, 4:1 were selected to co-culture. The function of osteoblasts was assessed by alkaline phosphatase (ALP) activity and osteocalcin (OC) assay. RESULTS: There was good compatibility when osteoblasts and vascular endothelial cells were co-cultured. The activity of ALP and OC in the group of ROB and RVEC co-cultured in ratio of 2:1 was higher than that of the other three groups. CONCLUSION: In direct co-culture system in vitro, RVEC can improve activity of ROB. |
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| Keywords: | osteoblast vascular endothelial cell alkaline pho sphatase osteocaclin |
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