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冬眠合剂对严重烫伤大鼠肺损伤的保护作用
引用本文:乔亮,YUAN Ke-jian,杨惠忠,DONG He-liang,王文奎,许伟石.冬眠合剂对严重烫伤大鼠肺损伤的保护作用[J].中华烧伤杂志,2008,24(4).
作者姓名:乔亮  YUAN Ke-jian  杨惠忠  DONG He-liang  王文奎  许伟石
作者单位:1. 上海交通大学医学院附属瑞金医院烧伤科,200025
2. Department of Burns, Ruijin Hospital, Medical College of Shanghai Jiaotong University, Shanghai 200025, P. R. China
摘    要:目的 观察早期应用冬眠合剂对严重烫伤大鼠肺损伤是否具有保护作用. 方法建立Ⅲ度烫伤大鼠模型,随机分为冬眠合剂组和对照组(未用冬眠合剂),每组36只.分别在伤后3、5、7、10 d测定大鼠动脉血氧分压,检测肺组织中过氧化物酶(MPO)活性、丙二醛(MDA)含量、肿瘤坏死因子α(TNF-α)及γ干扰素(IFN-γ)蛋白表达,动态观察伤后肺组织病理学变化. 结果与对照组大鼠伤后3 d动脉血氧分压(8.86±0.23)kPa(1 kPa=7.5 mm Hg)比较,冬眠合剂组该时相点明显升高(12.58±0.41)kPa,P<0.01].冬眠合剂组大鼠伤后各时相点肺组织MPO活性和MDA含量下降(P<0.05或P<0.01);伤后3、5、7 d肺组织TNF-α表达显著下调(P<0.05或P<0.01),伤后5、7、10 d肺组织IFN-γ的表达亦显著下调(P<0.01).冬眠合剂组大鼠肺泡间质水肿减轻,炎性细胞浸润减少. 结论大鼠烫伤后及时复苏并给予冬眠合剂,能适度抑制机体应激反应并下调促炎因子表达,改善早期肺功能.

关 键 词:烧伤  应激    呼吸窘迫综合征  成人  冬眠合剂

Protective effect of lytic cocktail (inhibition of stress) on lung injury in severe bum rat
QIAO Liang,YUAN Ke-jian,YANG Hui-zhong,DONG He-liang,WANG Wen-kui,XU Wei-shi.Protective effect of lytic cocktail (inhibition of stress) on lung injury in severe bum rat[J].Chinese Journal of Burns,2008,24(4).
Authors:QIAO Liang  YUAN Ke-jian  YANG Hui-zhong  DONG He-liang  WANG Wen-kui  XU Wei-shi
Abstract:Objective To investigate the protective effect of inhibition of stress (lytic cocktail) on lung injury in severe bum rats at early stage. Methods Sprague-Dawley rats inflicted with 30% TBSA full- thickness burn were randomly divided into A group ( n = 36, fluid resuscitation with administration of lyric cocktail) , B group( n = 36, fluid resuscitation only). Lung function was evaluated by partial pressure of ox- ygen ( PaO2 ) in arterial blood and histopathologic changes on 3, 5, 7, 10 post bum day (PBD). The levels of malonyldialdehyde (MDA) , myeloperoxidase (MPO) , tumor necrosis factor-α(TNF-α) and interferon-γ (IFN-γ) in lung tissue were measured at the same time points. Results The PaO2 level in A group on 3 PBD (12.58±0.41 kPa) was significantly higher than that in B group ( 8.86±0. 23 kPa, P < 0.01 ). Compared with those in B group, the levels of MDA and MPO were significantly decreased in A group at each time point( P <0.05 or0.01) ,the levels of TNF-α on 3,5,7 PBD ( P <0.05 or0.01)and IFN-γ on 5,7,14 PBD( P < 0. 01 ) were also decreased in A group. Swollen lung mesenchyme was alleviated,infiltra- tion of inflammatory cell was lessened in A group. Conclusion Lytic cocktail combined with immediate fluid resusitation can inhibit stess response, downregulate the expression of inflammatory factor, ameliorate lung function in severe burn rat at early stage.
Keywords:Bums  Stress  Lung  Respiratory distress syndrome  adult  Lyric cocktail
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