An autophagy gene, <Emphasis Type="Italic">MgATG5</Emphasis>, is required for cell differentiation and pathogenesis in <Emphasis Type="Italic">Magnaporthe oryzae</Emphasis> |
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Authors: | Jian-Ping Lu Xiao-Hong Liu Xiao-Xiao Feng Hang Min and Fu-Cheng Lin |
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Institution: | (1) College of Life Sciences, Zhejiang University, Yuhangtang Road 388, 310058 Hangzhou, China;(2) Key State Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Kaixuan Road 268, 310029 Hangzhou, China |
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Abstract: | Autophagy is a conserved degradation pathway that is involved in the maintenance of normal cell differentiation and development.
The Saccharomyces cerevisiae
ATG5 gene is an important component of the autophagy process. In this study, we identified MgATG5 as an autophagy-related gene in Magnaporthe oryzae that is homologous to ATG5. Using targeted gene replacement, an Mgatg5∆ mutant was generated and fungal autophagy was blocked. Cytological analysis revealed that the mutant had poor fungal morphogenic
development, including a shortened aerial hyphae lifespan, decreased conidiation and perithecia formation, delayed conidial
germination and appressorial formation, postponement of conidial cytoplasm transfer during appressorium formation, and reduction
in formation of the penetration peg. Turnover of endogenous matter in the Mgatg5∆ mutant was also affected, as demonstrated by defects in the formation of conidial lipid droplets, and in the degradation
of conidial glycogen deposits during appressorium formation. Lipid droplets and glycogen are necessary to generate adequate
turgor in appressoria for invading the host surface. As a result of the decreased appressorium turgor and differentiation
in the penetration peg, Mgatg5∆ pathogenicity was deficient in two host plants tested. The developmental and pathogenic phenotypes were restored by the
introduction of an intact copy of MgATG5 into Mgatg5∆, demonstrating that the MgATG5 deletion was responsible for the cellular defects. Taken together, these findings suggest that autophagy promotes cell differentiation
through turnover of endogenous matter during fungal development, and is thus essential for the pathogenicity of the rice blast
fungus.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
J.-P. Lu and X.-H. Liu contributed equally to this work and are regarded joint first authors. |
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Keywords: | MgATG5 Autophagy Magnaporthe oryzae Cell differentiation Pathogenicity |
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