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| 大鼠脑缺血再灌注免疫组化结果观察方法的改进 | |
| 引用本文： | 边立忠,李琴,刘成玉,郭云良. 大鼠脑缺血再灌注免疫组化结果观察方法的改进[J]. 齐鲁医学杂志, 2004, 19(6): 519-520 |
| 作者姓名： | 边立忠 李琴 刘成玉 郭云良 |
| 作者单位： | 1. 山东省淄博市齐都医院检验科,山东,淄博,255400 2. 山东省荣成市第二人民医院神经内科 3. 青岛大学医学院诊断学教研室 4. 青岛大学医学院脑血管病研究所 |
| 基金项目： | 山东省自然科学基金资助项目 (Y2 0 0 1C0 4) |
| 摘 要： | ①目的：探讨一种改进的大鼠脑缺血再灌注免疫组化结果观察方法。②方法：用线栓法制备大鼠局灶性脑缺血再灌注模型，参照临床症状评分对比分析阳性颗粒计数法和阳性累积分法的观察结果。③结果：阳性累积分法所获得结果比阳性颗粒计数法所获得结果与大鼠临床症状评分具有更高的一致性。④结论：阳性累积分法结果准确，优于阳性颗粒计数法，更适合大鼠脑缺血再灌注免疫组化结果的观察分析。 |
| 关 键 词： | 脑缺血 再灌注损伤 免疫组织化学 大鼠 大脑中动脉 |
| 文章编号： | 1008-0341(2004)06-0519-02 |
| 修稿时间： | 2003-09-03 |
AN IMPROVED OBSERVATION OF IMMUNOHISTOCHEMICAL RESULTS OF CEREBRAL ISCHEMIA-REPERFUSION EXPERIMENT IN RATS |
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| BIAN Li-zhong,LI Qin,LIU Cheng-yu,et al. AN IMPROVED OBSERVATION OF IMMUNOHISTOCHEMICAL RESULTS OF CEREBRAL ISCHEMIA-REPERFUSION EXPERIMENT IN RATS[J]. Medical Journal of Qilu, 2004, 19(6): 519-520 | |
| Authors: | BIAN Li-zhong LI Qin LIU Cheng-yu et al |
| Abstract: | Objective To explore a new method to observe the immunohistochemical results of cerebral ischemia-reperfusion experiment in rats. Methods A focal cerebral ischemia-reperfusion model in rats was made by inserting a string into the middle artery. The differences between the granule-positive data and the accumulation-positive data were compared. Results The results from accumulation-positive method were more closely related with rats clinical symptoms than those from granule-positive method. Conclusion The accumulation-positive method is an accurate measurement in evaluating immunohistochemical results of cerebral ischemia-reperfusion experiment in rats. |
| Keywords: | cerebral ischemia reperfusion injury immunohistochemistry rats middle cerebral artery |
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