芳香化酶P450在子宫内膜癌中的表达及其意义

 目的 探讨芳香化酶P450（P450arom）在正常子宫内膜、增生子宫内膜以及子宫内膜癌中的表达，探讨P450arom在子宫内膜癌发生和发展中的作用。方法 实验分成三组：子宫内膜腺癌组标本50份、子宫内膜增生症组内膜标本27份、正常子宫内膜组标本20份。采用免疫组化（ABC）方法分别测定3组标本中P450arom的表达。结果 P450arom在子宫内膜癌、子宫内膜增生症内膜腺上皮中均阳性表达，两者比较差异无统计学意义（P＞0.05）。P450arom 在正常子宫内膜中无表达。P450arom在子宫内膜腺癌癌组织间质细胞中表达21例（42 %），间质细胞阳性表达与手术病理期别及病理分级密切相关，而与肌层浸润深度、淋巴结转移无关。结论 P450arom在子宫内膜癌发生、发展中起重要作用。子宫内膜腺癌癌组织间质细胞P450arom表达与肿瘤发展及预后密切相关。

Expression of P450 aromatase in endometrial carcinoma

Objective To investigate the funcion of P450 aromatase in normal，hyperplasia en－dometrium and endometrioid endometrial carcinoma. Methods There were three experimental groups: en－dometrial carcinoma（50 cases）, endometrial hyperplasia（27 cases）, and normal endometrium（20 cases）. P450arom was detected by using immunohistochemical methods (ABC method) with specific antibodies. Re－sults P450aroma was expressed in the glandular epithelial of hyperplasia and malignant endometrium, there was no difference between two groups(P ＞0.05). Immunohistochemical staining for intratumoral aromatase in endometrial cancer was positive, 21(42 %) in stromal cells. The expression of P450 aromatase in stromal cells correlated positively with advanced surgical stage and histologic grade, indepedent on muscular invasion and lymph node metastasis. Conclusion Aromatase P450 play an important function in establishment and devel－opment of the endometrioid endometrial cancer. The expression of P450 aromatase in intratumoral stromal cells correlated positively with the development and prognosis of endometrioid endometrial cancer.