HPLC测定三黄汤中4种成分含量

目的 建立高效液相色谱法测定三黄汤中4种重要活性成分(绿原酸、盐酸药根碱、盐酸小檗碱和盐酸巴马汀)的含量。 方法 采用Agilent C₁₈色谱柱(4.6 mm×200 mm，5 μm)，流动相为1%醋酸甲醇溶液-1%醋酸溶液梯度洗脱；检测波长：329 nm；流速：1.0 mL·min^-1。 结果 绿原酸、盐酸药根碱、盐酸小檗碱和盐酸巴马汀的线性范围分别为0.5~4.0 μg·mL^-1(r=0.999 1)，0.5~4.0 μg·mL^-1(r=0.999 7)，0.5~12.0 μg·mL^-1(r=0.999 3)，0.3~4.0 μg·mL^-1(r=0.999 9)。平均回收率分别为99.0%(RSD=1.13%)，99.3%(RSD=1.20%)，99.0%(RSD=0.27%)，99.8%(RSD=0.88%)。日内、日间RSD均<2%。 结论 本方法准确、灵敏、重复性好，可同时测定三黄汤中4种重要化学成分的含量。

Determination of Four Components in Sanhuang Decoction by HPLC

OBJECTIVE To establish an HPLC method for simultaneous determination of four important active components in Sanhuang decoction. METHODS An Agilent C18 column （4.6 mm×200 mm, 5 μm） was used with the mobile phase of 1% acetic acid methanol solution-1% acetic acid by gradient dilution, at the flow rate of 1.0 mL·min^-1 and detection wavelength of 329 nm. RESULTS The linear calibration curves of chlorogenic acid, jatrorrhizine hydrochloride, berberine hydrochloride and palmatine hydrochloride were obtained in the ranges of 0.5-4.0 μg·mL^-1（r=0.999 1）, 0.5-4.0 μg·mL^-1 （r=0.999 7）, 0.5-12.0 μg·mL^-1（r=0.999 3）, 0.3-4.0 μg·mL^-1 （r=0.999 9）, respectively. Their average recoveries （n=3） for the assay were 99.0%（RSD=1.13%）,99.3%（RSD=1.20%）,99.0%（RSD=0.27%） and 99.8%（RSD=0.88%）, respectively. The intra- and inter-day RSDs were all less than 2.0%. CONCLUSION This method is accurate, sensitive and suitable for quality control of Sanhuang decoction.