激惹诱导心律失常大鼠血清中NPY、CST的变化及其中药影响的作用机制

目的:探讨激惹诱导心律失常大鼠血清中NPY、CST的变化及中药影响的作用机制。方法:选取雄性SD大鼠48只,随机分为模型对照(A)、中药对照(B)、β受体阻滞剂组(C)和造模前对照(D)等4组。A、B、C组分别与造模前12只大鼠中血清NPY、CST水平做比较分析。利用酶联免疫吸附法(ELISA)观察造模前后各组大鼠血清神经肽Y(NPY)和儿茶酚胺抑素(CST)的含量,结果:模型组血清NPY、CST含量明显高于造模前对照组的(P<0.05),中药组NPY、CST水平与模型组比较明显下降、有明显差异(P<0.05),有统计学意义,中药组NPY、CST水平较西药组仍有下降,有明显差异(P<0.05)。结论:神经肽Y与儿茶酚胺抑素均与心律失常密切相关。中药明显降低大鼠血清中神经肽含量,升高儿茶酚胺抑素的表达水平,中药疏肝法对心律失常有一定的调节作用。

Induced Arrhythmia in Rats the Change of NPY and CST in Serum by Irritating and the Mechanism of Effect of Traditional Chinese Medicine

Objective ： to investigate that Induced arrhythmia in rats the change of NPY and CST in serum by irritating and the mechanism of effect of traditional Chinese medicine. Methods ： Male Wistar rats were randomly divided into model control group （A）, traditional Chinese medicine control group （B）, blockers control group （C） and irritability stimulated control group （D）. Group A, B and C were comparing the serum NPY, CST level to the 12 rats before building model respectively and making a comparative analysis. The use of enzyme - linked immunosorbent assay （ELISA） was observed in each group before and after modeling serum neuropeptide Y （NPY） and catecholamine endostatin （CST） of content. Results ： The NPY, CST in serum of model group were significantly higher than the control group before modeling （ P 〈 0.05 ）. the NPY, CST in TCM group levels higher than western medicine group levels and increased significantly, there are significant differences （ P 〈 0. 05 ）. Conclusion ： Neuropeptide Y and catecholamines endostatin were closely associated with abnormal heart rate. Chinese herbs can inhibit neuropeptide serum levels, increased expression levels of catecholamines endostatin. （P 〈 0.01 ）. Chinese herbs and therapy of regulating the liver have a regulatory role to arrhythmia.