| 多元药物抗性基因(mdr-1)在白血病细胞中的表达 |
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| 引用本文： | 王申五,白桃,刘晓荣,单福香,江滨,赵实诚.多元药物抗性基因(mdr-1)在白血病细胞中的表达[J].北京大学学报(医学版),1992(3). |
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| 作者姓名： | 王申五 白桃 刘晓荣 单福香 江滨 赵实诚 |
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| 作者单位： | 北京医科大学血液病研究所
(王申五，白桃，刘晓荣，单福香，江滨)，北京医科大学血液病研究所(赵实诚) |
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| 摘 要： | 多元药物抗性基因(multidrug resistance gene,mdr-1)编码 P 170膜蛋白,可使细胞对多种结构不同的细胞毒药物产生抗性。本文以 mdr-1 cDNA为探针,做白细胞总 RNA 斑点杂交分析了5例髓性白血病人mdr-1的mRNA水平。说明mdr-1基因在此类患者的白细胞中的表达有明显差异,同一病人治疗前后也有改变。所建立的观查临床标本中基因表达水平的方法,具广泛的应用价值。
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| 关 键 词： | mdr-1 RNA斑点杂交 髓性白血病 |
EXPRESSION OF MDR-1 IN LEUKEMIC CELLS |
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| Wang Shenwu,et al Institute of Hematology.EXPRESSION OF MDR-1 IN LEUKEMIC CELLS[J].Journal of Peking University:Health Sciences,1992(3). |
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| Authors: | Wang Shenwu Institute of Hematology |
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| Abstract: | P-glycoprotein (Pgp; Mr=170,000) is encoded by a family of genes-Multidrug resistance gene.The Pgp bas been demonstrated to mediate resistance to multiple structurally dissimilar drugs, whichfuctions as an energy-dependent efflux pump so that a cell with high level of mdr expression canmore effectively eliminate cytotoxic drugs. In this report, a simplified method for analysis of clinicalsamples and assess the level of gene expression was set up. Furthermore, by using ~(32)P labelled mdr-1cDNA as the probe and RNA dot blotting the mdr-mRNAs from 5 cases of myeloblastic leukemiacells were analysed. It was shown that the level of mdr-1 expression in different myeloleukemic cellswas various and reduced in one case after remission. The established method for mRNA analysis couldbe generalized for evaluating the level of mRNA in clinical samples. |
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| Keywords: | mdr RNA Dot blotting Myeloblastic leukemia |
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