| 用于蛋白质间相互作用研究的分析技术 |
| |
| 引用本文： | 徐志洲,梁翠荣,陈海,田浤,高向东. 用于蛋白质间相互作用研究的分析技术[J]. 药学进展, 2010, 34(4): 152-158. DOI: 10.3969/j.issn.1001-5094.2010.04.002 |
| |
| 作者姓名： | 徐志洲 梁翠荣 陈海 田浤 高向东 |
| |
| 作者单位： | 1. 山东省药品检验所,山东,济南,250101
2. 中国药科大学生命科学与技术学院,江苏,南京,210009 |
| |
| 摘 要： | 概述近年来用于蛋白质间相互作用研究的分析技术,如酵母双杂交系统、荧光共振能量转移技术、表面等离子体共振技术、蛋白质芯片技术和生物信息学方法等,分别介绍其应用原理和特点,为蛋白质间相互作用研究及构建蛋白质相互作用图谱提供理论依据。
|
| 关 键 词： | 蛋白质间相互作用 分析技术 酵母双杂交系统 荧光共振能量转移 表面等离子共振 蛋白质芯片 生物信息学 |
Overview of Analytical Techniques for Protein-protein Interaction Studies |
| |
| XU Zhi-zhou,LIANG Cui-rong,CHEN Hai,TIAN Hong,GAO Xiang-dong. Overview of Analytical Techniques for Protein-protein Interaction Studies[J]. Progress in Pharmaceutical Sciences, 2010, 34(4): 152-158. DOI: 10.3969/j.issn.1001-5094.2010.04.002 |
| |
| Authors: | XU Zhi-zhou LIANG Cui-rong CHEN Hai TIAN Hong GAO Xiang-dong |
| |
| Affiliation: | XU Zhi-zhou1,LIANG Cui-rong1,CHEN Hai2,TIAN Hong2,GAO Xiang-dong2(1.Sh,ong Institute for Drug Control,Jinan 250101,China,2.School of Life Science , Technology,China Pharmaceutical University,Nanjing 210009,China) |
| |
| Abstract: | Analytical techniques for protein-protein interaction studies,such as the yeast two-hybrid system,fluorescence resonance energy transfer,surface plasmon resonance,protein chip and bioinformatics method,were summarized and their principles and characteristics were introduced,which would be beneficial for protein-protein interaction analysis and mapping. |
| |
| Keywords: | protein-protein interaction analytical technique yeast two-hybrid system fluorescence resonance energy transfer surface plasmon resonance protein chip bioinformatics |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
