CCR5和CD30在自身免疫性甲状腺疾病外周淋巴细胞上的表达

目的:探讨自身免疫性甲状腺疾病(AITD)中辅助性T淋巴细胞(Th1/Th2)的平衡偏移及其在发病中的作用。方法:选择21例初发Graves病(GD组),21例桥本甲状腺炎(HT组)和45名健康人(对照组)为研究对象,应用流式细胞仪技术检测外周血淋巴细胞中CD4 CCR5 和CD4 CD30 细胞,以其各自百分比和平均荧光强度(MFI)作为反映Th1/Th2免疫应答强度的指标。结果:与对照组相比,GD组CD4 CD30 细胞升高(P<0.05),而CCR5 细胞MFI明显减低(P<0.01);HT组CD4 CCR5 和CD4 CD30 细胞均升高(P<0.05)。GD组中CD30 细胞MFI与TRAb浓度正相关,与FT3、FT4、TSH均无相关性。结论:GD的发生可能与Th2优势应答密切相关;Th1和Th2细胞可能共同参与了HT的发病。

Expression of CCR5 and CD30 molecules in peripheral blood lymphocytes of patients with autoimmune thyroid disease

Objective: To explore the T helper cells (Th1/Th2) bias in autoimmune thyroid disease (AITD) and its effect on the development of AITD. Methods: The percentage and mean fluorescence intensity (MFI) of CCR5-positive and CD30-positive cells were determined by using flow cytometry in 21 patients with Graves' disease (GD group),21 patients with Hashimoto thyroiditis (HT group),and 45 healthy subjects (control group). Results: Compared with the control group,the percentage of CD30-positive cells significantly increased (P < 0.05) and the MFI of CCR5-positive cells significantly decreased (P < 0.01) in GD group. The percentages of both CCR5-positive and CD30-positive cells significantly increased in HT group (P < 0.05) . The MFI of CD30-positive cells were positively correlated with the level of thyroid stimulating hormone receptor antibody. Conclusion: GD is a Th2-dominated disease. Both Th1 and Th2 cells may be involved in the pathogenesis of HT.