DFU对骨肉瘤细胞增殖和侵袭能力的影响

目的 观察选择性环氧合酶-2抑制剂DFU对人骨肉瘤细胞株MG-63增殖和侵袭能力的影响及其对Ang-2基因表达的调控.方法 体外培养骨肉瘤细胞株MG-63,免疫荧光法检测Ang-2在MG-63细胞中的表达,应用噻唑蓝(MTT)比色法和形态学检测研究不同浓度DFU对骨肉瘤细胞株MG-63的生长抑制作用,Boyden小室体外侵袭实验检测其对骨肉瘤细胞侵袭能力的影响,RT-PCR法分析DFU对骨肉瘤细胞株MG-63中Ang-2基因表达的影响.结果 免疫荧光染色结果显示Ang-2在骨肉瘤细胞株MG-63中呈阳性染色,MTT法和形态学检测提示DFU可抑制骨肉瘤细胞株MG-63的增殖,并具有剂量依赖性,在DFU浓度为200 μmol/L时,对细胞生长有明显的抑制作用.Boyden小室体外侵袭实验显示DFU可降低骨肉瘤细胞的侵袭能力,在DFU浓度为200 μmol/L时,作用最显著.逆转录-聚合酶链反应(RT-PCR)分析显示经DFU作用后的骨肉瘤细胞株MG-63表达Ang-2较用药前明显下降.结论 DFU对骨肉瘤细胞株MG-63的增殖有抑制作用,其可能通过抑制Ang-2的表达,降低其侵袭能力.

The inhibitory effects of DFU on proliferation and invasion of osteosarcoma cells

Objective To investigate the inhibitory effects of DFU, a Cyclooxygenase-2-selected-inhibitor,on proliferation and invasion of osteosarcoma cells line MG-63,and the influences of DFU on the expression of angiopoietin-2(Ang-2) in MG-63 cells. Methods Osteosarcoma cells line MG-63 was cul-tured in vitro. The expression of Ang-2 in MG-63 was observed by immunofluorescence. Methyl thiazolyl tetrazolium (MTT) and morphology were used to study the inhibitory effects of DFU with different concen-tration on osteosarcoma cells line MG-63. Boyden chamber in vitro invasion assay was applied to observe the changes of invasion capability. The expression of Ang-2 of osteosarcoma cells line MG-63 was analysed by RT-PCR. Results Positive staining was seen in the cells line MG-63 by immunofluorescence. The in-hibition rate of osteosarcoma cells line MG-63 was higher when treated with DFU and the rate reached the peak when the concentration of DFU was 200 μmoL/L. The invasion ability was remarkably decreased after treated with DFU. Ang-2 mRNA expression of osteosarcoma cells line MG-63 declined obviously than be-fore medication. Conclusion DFU has lethal effect on osteosarcoma cells line MG-63. It may inhibit the proliferation and invasion of osteosarcoma cells line MG-63 by inhibiting the expression of Ang-2.