枸杞多糖粗品抗突变作用的研究

目的研究枸杞多糖粗品（LBPc）对遗传损伤的保护作用。方法采用微核试验、Ames试验和DNA修复试验方法评价不同浓度的枸杞糖粗品的抗突变作用。结果不同浓度的LBPc可使醋酸铅诱导的小鼠骨髓多染红细胞微核率明显降低，3个试验组的微核率分别为4．5‰、3．7‰、1．9‰，LBPc高剂量组（20mg/kg）与阳性对照组（5．3‰）相比，差异有非常显著性（P〈0．01），并呈剂量-反应关系；Ames试验结果表明，加入不同浓度的LBPc后，其抗突变作用由弱变强；职业人群服用LBPc1周后，其DNA修复速度明显提高（P〈0．01）。结论LBPe具有明显的抗突变活性。

Study of the Anti-Mutagenic Effect of the crude Lycium barbarum polysaccharide

[Objective]To study the protective effect ofthe crude Lycium barbarum polysaccharide against genetic damages.[Methods]Micronucleus test,Ames test and DNA repair test were done to evaluate the anti-mutagenic effect of the crude Lycium barbarum polysaccharide with different concentrations.[Results]the crude Lycium barbarum polysaccharide with different concentrations can reduce the micronucleus rates of the mice's marrow cells induced by acetic lead,the micronucleus rates of the 3 groups were 4.5%,3.7% and 1.9% respectively.Great significance was found between the high-dose group(20mg/kg) and the positive control group (5.3%)(P<0.01).Ames test showed an anti-mutagenicity ranging from weak to strong depending on the concentration of the test samples.The DNA repair speed increased rapidly after the intake ofthe crude Lycium barbarum polysaccharide(P<0.01).[Conclusion]the crude Lycium barbarum polysaccharide is proved to have obvious anti-mutagenicity.