补肾中药对溴隐亭致流产鼠模型中蜕膜CD56+NK细胞表面CD69和CD94表达的影响

为了观察补肾中药是否能影响蜕膜CD5 6 + NK细胞比例及其表面CD6 9、CD94的表达 ,从而进一步阐明补肾中药对母胎界面免疫的影响机制。SD大鼠于孕 6～ 8d皮下注射溴隐亭 0 3mg/kg·d ,建立改良的溴隐亭致SD大鼠流产模型 ,随机分为三组。A组为模型组 ;B、C组在孕 1～ 1 1d分别给予大剂量中药 (4 5g/kg·d)、P(8mg/kg·d) ,另设正常孕鼠对照 (D组 )。孕 1 2d处死 ,取蜕膜组织 ,分离成单个细胞悬液 ,用流式细胞分析技术观察 4组蜕膜CD5 6 + NK细胞的量及蜕膜NK细胞表面CD6 9、CD94的表达差异。结果发现 :B组 (P <0 0 5 )、C、D组 (P <0 0 1 )妊娠率与A组相比增加 ,并且有差异 ;B、C组蜕膜CD5 6 + NK细胞比例与A组相比明显增加 ,与D组无差异 ;各组蜕膜CD5 6 + NK细胞表面CD6 9表达无差异 ;B、C、D组CD94的表达与A组相比显著增加 ;A组蜕膜CD5 6 + NK细胞表达CD6 9与CD94相比明显增加 ,B、C组相反。因此 ,补肾中药能影响蜕膜CD5 6 + NK细胞表面CD6 9、CD94的表达 ,可能通过增加CD94的表达 ,抑制CD6 9从而进一步抑制NK细胞的活性 ,起到保胎作用

Effects of Kidney-Replenishing Herbs on the Expression of CD69 and CD94 on the CD56+ Decidual NK Cells in Improved Bromocriptine-Induced Abortion

To observe the effects of kidney replenishing herbs on the expressions of CD69 and CD94 on the CD56 + decidual NK cells in the improved bromcriptine induced abortion Pregnant SD rats were divided into 4 groups in random,in which rats in group A were injected subcutaneously with bromocriptine (0 3 mg/kg·d) during 6～8 days of pregnancy;rats in group B were introduced orally with powder of Chinese herbs (4 5 g/kg·d) during 1～11 days of pregnancy,rats in group C were injected with progesterone (8 mg/kg·d) during 1～11 days of pregnancy;rats in group B and C were also injected subcutaneously with bromocriptine(0 3mg/kg·d) during 6～8 days of pregnancy,those in group D were normal pregnant rats At 12 th day of pregnancy,all these rats were killed and the decidual tissues were taken and identified morphologically,and the decidual cell suspensions were then made The numbers and the surface expressions of CD69 and CD94 on the cells of these four groups of rats were determined by means of flow cytometry analysis It was found that the percentage of CD56 + decidual NK cells was higher in group B,C,D groups than in group A A significant elevation in the expressions of CD94 on CD56 + NK cells was found in B,C,D groups in comparison with that of group A,but the expression of CD69 showed no significant difference in these four groups of rats For group A,the expression of CD69 on the CD56 + decidual NK cells was higher than that of CD94 It concludes that kidney replenishing herbs may affect the percentage of CD56 + decidual NK cells and the expressions of CD69 and CD94 on these cells