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| 重组人白细胞介素10对角朊细胞增殖及产生细胞因子的影响 | |
| 引用本文： | 陈飞虎,陈敏珠,李俊,汪光宝,智强.重组人白细胞介素10对角朊细胞增殖及产生细胞因子的影响[J].中国临床药理学与治疗学,2004,9(12):1377-1380. |
| 作者姓名： | 陈飞虎 陈敏珠 李俊 汪光宝 智强 |
| 作者单位： | 1. 安徽医科大学药学院,合肥,230032,安徽 2. 安徽医科大学临床药理研究所,合肥,230032,安徽 3. 安徽桑尼生物技术研究所,合肥,230032,安徽 |
| 摘 要： | 目的：研究重组人白细胞介素10(rhIL-10)对无血清培养的角朊细胞增殖和产生细胞因子的影响，探讨其治疗银屑病的作用机制。方法：用四甲基偶氮唑蓝比色法(MTT)测定其对细胞增殖的影响；小鼠胸腺细胞增殖法检测白细胞介素1(IL-1)；ELISA法检测白细胞介素6(IL-6)、白细胞介素8(IL-8)。结果：重组人白细胞介素10抑制角朊细胞增殖与IL-1、IL-6及IL-8的分泌，并呈剂量依赖关系。结论：重组人白细胞介素10抑制角朊细胞与细胞因子分泌，可能是治疗银屑病的作用机理之一． |
| 关 键 词： | 重组人白细胞介素10 角朊细胞 细胞因子 银屑病 |
| 文章编号： | 1009-2501(2004)12-1377-04 |
| 修稿时间： | 2004年7月8日 |
Effects of recombinant human interleukin-10 (rhIL-10) on proliferation and cytokines production of normal human keratinocytes |
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| CHEN Fei-Hu,CHEN Ming-Zhu,LI Jun,ZHI Qiang.Effects of recombinant human interleukin-10 (rhIL-10) on proliferation and cytokines production of normal human keratinocytes[J].Chinese Journal of Clinical Pharmacology and Therapeutics,2004,9(12):1377-1380. | |
| Authors: | CHEN Fei-Hu CHEN Ming-Zhu LI Jun ZHI Qiang |
| Institution: | CHEN Fei-Hu,CHEN Ming-Zhu1,LI Jun,ZHI Qiang2 Department of Pharmacy,1 Department of Clinical Pharmacology,Anhui Medical University,Hefei 230032,Anhui,China, 2 Anhui Nisang Institute of Biology T echnology,Hefei 230032,Anhui,China |
| Abstract: | AIM: To investigate the effects of recombinant human in terleukin-10 (rhIL-10) on proliferation and cytokines production of serum-fre e cultured normal human keratinocytes stimulated by PMA and lipopolysaccharides( LPS). METHODS: The influences of rhIL-10 on the proliferation o f serum-free cultured normal human keratinocytes were assayed by MTT. Interleuk in-1, interleukin-6, and interleukin-8 was detected by Enzyme-linked immunos orbent assay (ELISA). RESULTS: The rhIL-10 significantly inhibi ted the proliferation and the production of IL-1, IL-6 and IL-8 by KC which w as activation by PMA/LPS with dose-dependent manner. CONCLUSION: The rhIL-10 inhibits the proliferation and cytokines production of s erum-free cultured normal human keratinocytes. The anti-psoriatic effects of r hIL-10 may be related to inhibiting hyperplasia of epidermal cells, promoting t he formation of granular cells and possessing combined anti-inflammatory and im munomodulatory activity. |
| Keywords: | recombinant human interleukin-10 keratinocytes cyto kines psoriatic |
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