| 肺腺癌A549细胞抗失巢凋亡相关基因的确认及C8orf4基因的表达 |
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| 引用本文： | 李文海,胡运生,王小平,雷杰,苏凯,李小飞. 肺腺癌A549细胞抗失巢凋亡相关基因的确认及C8orf4基因的表达[J]. 现代肿瘤医学, 2011, 19(12): 2404-2407. DOI: 10.3969/j.issn.1672-4992.2011.12.13 |
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| 作者姓名： | 李文海 胡运生 王小平 雷杰 苏凯 李小飞 |
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| 作者单位： | 1. 第四军医大学唐都医院胸腔外科,陕西,西安,710038
2. 第四军医大学唐都医院骨科,陕西,西安,710038 |
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| 基金项目： | 陕西省科学技术研究发展计划基金资助项目 |
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| 摘 要： | 目的:比较抗失巢凋亡及正常贴壁生长肺腺癌A549细胞基因组表达差异,从中筛选肺癌抗失巢凋亡相关基因,并探讨C8orf4基因的高表达及其对失巢凋亡的正性调剂作用意义。方法:建立抗失巢凋亡肺癌A549细胞系;用基因芯片技术检测其与正常贴壁生长A549细胞的差异基因,利用NCBI Pubmed数据库筛选出肺癌细胞抗失巢凋亡相关的基因,找出差异表达倍数最大的基因,运用逆转录聚合酶链反应(RT-PCR)技术及蛋白质印迹技术测定其表达。结果:共检测到表达差异的基因745个,筛选出63个与肺癌细胞抗失巢凋亡相关的基因,C8orf4基因差异表达倍数最大,在脱落培养A549细胞中C8orF4基因及其编码蛋白表达明显高于贴壁培养A549细胞,P<0.02。结论:基因芯片技术为筛选肺癌抗失巢凋亡相关基因提供了有效方法,C8ORF4基因可能参与调控肺癌细胞抗失巢凋亡过程。
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| 关 键 词： | 肺癌 抗失巢凋亡 肺癌细胞系A549 C8orf4 |
Investigation on aniokis-resistantce associated gene and expression of C8orf4 gene in lung cancer A549 cell line |
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| LI Wenhai,HU Yunsheng,WANG Xiaoping,LEI Jie,SU Kai,LI Xiaofei. Investigation on aniokis-resistantce associated gene and expression of C8orf4 gene in lung cancer A549 cell line[J]. Journal of Modern Oncology, 2011, 19(12): 2404-2407. DOI: 10.3969/j.issn.1672-4992.2011.12.13 |
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| Authors: | LI Wenhai HU Yunsheng WANG Xiaoping LEI Jie SU Kai LI Xiaofei |
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| Affiliation: | LI Wenhai1,HU Yunsheng2,WANG Xiaoping1,LEI Jie1,SU Kai1,LI Xiaofei1 1 Department of Thoracic Surgery,Tangdu Hospital,Fourth Military Medical University,Shaanxi Xi'an 710038,China,2 Department of Orthopaedics Surgery |
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| Abstract: | Objective:To screen anoikis resistance-associated genes through gene array and investigate the expression and clinical significance of C8orf4 gene by comparing normal cultured and suspension cultured A549 cell line.Methods: The expressions of genes in suspension cultured and attachment cultured A549 cell line were compared by gene array,and NCBI Pubmed database was used to screen the genes which had correlations with anoikis resistance in the chip.To select the most differently-expressing gene among these g... |
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| Keywords: | pulmonary carcinoma anoikis resistant lung cancer cell A549 C8orf4 |
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