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| RT-PCR法检测尿脱落细胞中XIAP的表达在膀胱癌诊断中的价值 | |
| 引用本文： | 杨光天,赵海岩,温峰,杨晋,刘毅,方毅.RT-PCR法检测尿脱落细胞中XIAP的表达在膀胱癌诊断中的价值[J].现代肿瘤医学,2011,19(12):2477-2479. |
| 作者姓名： | 杨光天 赵海岩 温峰 杨晋 刘毅 方毅 |
| 作者单位： | 1. 徐州医学院附属连云港医院泌尿外科,江苏,连云港,222002 2. 徐州医学院附属连云港医院实验中心,江苏,连云港,222002 3. 徐州医学院附属连云港医院病理科,江苏,连云港,222002 |
| 基金项目： | 连云港市医学重点人才科研课题 |
| 摘 要： | 目的:比较膀胱癌患者尿液脱落细胞中XIAP表达的RT-PCR检测法和常规尿脱落细胞病理学检测在膀胱癌诊断中的临床价值。方法:采用逆转录聚合酶链反应技术(RT-PCR)检测51例膀胱尿路上皮癌患者尿液脱落细胞中XIAP-mRNA的表达,同时行常规尿脱落细胞病理学检测,20例非肿瘤人员作为对照组。结果:实验组51例尿脱落细胞XIAP-mRNA RT-PCR检测阳性27例(53%),尿脱落细胞学病理学检测阳性12例(24%),对照组20例尿脱落细胞XIAP-mRNA检测阳性1例(5.0%),对照组尿脱落细胞病理学检测阳性0例(0%)。实验组RT-PCR检测膀胱尿路上皮癌患者尿脱落细胞中XIAP表达的敏感性高于尿脱落细胞病理学检测,差异有极显著统计学意义(P<0.01),实验组RT-PCR检测膀胱尿路上皮癌患者尿中XIAP表达的敏感性显著高于非肿瘤对照组,差异有极显著统计学意义(P<0.01)。结论:膀胱尿路上皮癌患者尿脱落细胞中XIAP表达的RT-PCR检测法较常规尿脱落细胞病理学检测更敏感,临床上作为膀胱癌的筛选方法,有一定的临床价值。 |
| 关 键 词： | 膀胱肿瘤 XIAP 诊断 逆转录聚合酶链反应(RT-PCR) |
Detection of XIAP in exfoliated urinary cells for diagnosis of bladder transitional cell carcinoma |
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| YANG Guangtian,ZHAO Haiyan,WEN Feng,YANG Jin,LIU Yi,FANG Yi.Detection of XIAP in exfoliated urinary cells for diagnosis of bladder transitional cell carcinoma[J].Journal of Modern Oncology,2011,19(12):2477-2479. | |
| Authors: | YANG Guangtian ZHAO Haiyan WEN Feng YANG Jin LIU Yi FANG Yi |
| Institution: | YANG Guangtian1,ZHAO Haiyan1,WEN Feng1,YANG Jin2,LIU Yi3,FANG Yi1 1 Department of Urology,2Test Center,3 Department of Pathology,Lianyungang Hospital Affiliated of Xuzhou Medical College,Jiangsu Lianyungang 222002,China |
| Abstract: | Objective:To compare the clinical value of XIAP detection in exfoliated urinary cells with exfoliated urine cytology in diagnosis of bladder transitional cell carcinoma.Methods: Expression of XIAP-mRNA in exfoliated urinary cells as well as exfoliative cytology were detected in 51 patients with bladder transitional cell carcinoma and 20 non-cancerous patients.Results: Of 51 patients 27(53%) with bladder transitional cell carcinoma showed positive XIAP in urine,while 12 of 51(24%)patients with bladder transi... |
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