| 单磷酸阿糖腺苷的离子对色谱法测定 |
| |
| 引用本文： | 徐明全,曾凡.单磷酸阿糖腺苷的离子对色谱法测定[J].中国医药工业杂志,2001,32(2):67-67,84. |
| |
| 作者姓名： | 徐明全 曾凡 |
| |
| 作者单位： | 广东省药物研究所, |
| |
| 摘 要： | 采用离子对色谱法测定单磷酸阿糖腺苷的含量。采用NovapakC18(150×3.9mm,4μm)反相柱，流动相为甲醇-水(含10mmol/L氢氧化四丁基铵和10mmol/LKH2PO4)(15∶85)，检测波长258nm。本方法的平均回收率为99.97%，RSD为0.63%。
|
| 关 键 词： | 单磷酸阿糖腺苷 离子对色谱法 测定 含量 DNA病毒抑制剂 |
| 文章编号： | 1001-8255(2001)02-0067-02 |
Determination of Ara-AMP
by Ion Pair HPLC |
| |
| Authors: | XU Ming-quan ZENG Fan |
| |
| Abstract: | A HPLC method was established for the determination of Ara-AMP, using Novapak C18 column (150×3.9 mm,4 μm), with the mobile phase of methanol-water (containing 10 mmol/L tetrabutylammonium hydroxide and 10 mmol/L KH2PO4) (15∶85). The flow rate was 1 ml/min and the UV detection wavelength was 258 nm. The calibration curve was linear in the range of 20～50 μg/ml (r=0.9998). The average recovery was 99.97% (RSD=0.63%). |
| |
| Keywords: | Ara AMP IP HPLC determination |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
