叔丁基对苯二酚对锰致神经细胞毒性的保护作用 |
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引用本文: | 李煌元,吴思英,林炜,周文华,张文昌,李涛,石年. 叔丁基对苯二酚对锰致神经细胞毒性的保护作用[J]. 中华劳动卫生职业病杂志, 2009, 27(10). DOI: 10.3760/cma.j.issn.1001-9391.2009.10.006 |
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作者姓名: | 李煌元 吴思英 林炜 周文华 张文昌 李涛 石年 |
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作者单位: | 1. 福州,350004,福建医科大学公共卫生学院,福建省重点建设大学环境与健康重点学科,环境与健康研究所毒理学与化学物安全性评价研究室,职业与环境卫生学系 2. 华中科技大学同济医学院公共卫生学院教育部环境与健康重点实验室,卫生毒理学系 |
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基金项目: | 福建省自然科学基金资助项目,国家自然科学基金资助项目,福建省教育厅基金资助项目 |
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摘 要: | 目的 研究叔丁基对苯二酚(tBHQ)对锰致大鼠肾上腺嗜铬细胞瘤(PC12)细胞毒性的保护作用.方法 以不同终浓度(300、600、900μmol/L)的MnCl2分别处理PC12细胞24、48、72 h,用噻唑蓝(MTT)法检测PC12细胞毒性.MnCl2组予600μmol/L MnCl2处理72 h;tBHQ组予40 μmol/L tBHQ处理细胞84h;tBHQ+MnCl2组予40μmol/L tBHQ预处理12h后,600 μmol/LMnCl2处理72 h,用MTT法检测细胞毒性,MnCl2处理剂量为300 μmol/L时用Annexin V-FITC/PI法流式细胞仪(FCM)检测细胞凋亡.结果 与对照组比较,300、600、900μmol/LMnCl2处理24、48、72h,细胞增殖相对比值降低,差异均有统计学意义(P<0.05,P<0.01);300、600、900 μmol/L MnCl2处理组各组间相互比较,有剂量一效应关系,差异均有统计学意义(P<0.01).与对照组比较,600 μmol/L MnCl2组抑制PC12细胞增殖,抑制率为40%,差异有统计学意义(P<0.01).与对照组及MnCl2组比较,tBHQ组和tBHQ+MnCl2组的细胞出现增殖效应,细胞增殖相对比为1.8,差异均有统计学意义(P<0.01).300μmol/L MnCl2处理组PC12细胞凋亡率明显高于对照组,差异有统计学意义(P<0.01),tBHQ+MnCl2组细胞凋亡率低于300μmol/L MnCl2处理组,差异有统计学意义(P<0.01),细胞凋亡抑制率61%.结论 锰可抑制PC12细胞增殖作用,可诱导细胞凋亡;tBHQ可减弱锰抑制PC12细胞的增殖作用并可削弱锰致PC12细胞凋亡的作用.
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关 键 词: | 锰 叔丁基对苯二酚 细胞毒性 免疫 PC12细胞 |
Protective effect of tert-butylhydroquinone on PC12 cells from neurotoxicity induced by manganese in vitro |
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Abstract: | Objective To investigate the protective effect of the tert-butylhydroquinone (tBHQ) on PC12 cells from neurotoxicity induced by manganese.Methods Cytoyoxicity of PC12 cells was measured by MTT assay,following the PC12 cells treatment with different concentrations of MnC12 ( 300,600,900 μmol/L) for 24,48 or 72 h.PC12 cells were pretreated with 40 μmol/L tBHQ for 12 h,followed by the treatment of 600 μmol/L or 300 μmol/L MnCl2 for 72 h.Cytotoxicity of PC12 cells was measured by MTT assay,and cell apoptosis was examined by the method of Annexin V-FITC/PI in flow cytometry(FCM).Results The proliferation of PC12 cells treated with 300,600,900 μmol/L MnCl2 was suppressed in the dose dependent pattern (P<0.01 ).Proliferation of PC12 cells treated with 600 μmol/L MnCl2 was suppressed to 40% of that in control group (P<0.01),but the proliferation rate of PC 12 cell pretreated with 40 μmol/L tBHQ was 180% of that in control group (P<0.01 ).Apoptotic rate of PC 12 cells treated with 300 μ mol/L MnCl2 was higher than the vehicle control group (P<0.01).Apoptotic rate of 40 μmol/L tBHQ pretreatment followed by 300 μmol/L MnCl2 treatmcnt was lower than that of MnCl2 treatment group (P<0.01).The inhibition rate of apoptosis was 61%.Conclusions Manganese may suppress PC12 cells proliferation and induce apoptosis,tBHQ can reduce PC12 cells proliferation suppressed by manganese and attenuate the apoptosis induced by manganese. |
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Keywords: | Manganese tert-Butylhydroquinone(tBHQ) Cytotoxicity immunologic PC 12 cells |
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