| 人甲状腺球蛋白的纯化和鉴定 |
| |
| 引用本文： | 陈广洁,席晔斌,王保国,周文达,李伟毅. 人甲状腺球蛋白的纯化和鉴定[J]. 免疫学杂志, 1999, 15(1): 61-63 |
| |
| 作者姓名： | 陈广洁 席晔斌 王保国 周文达 李伟毅 |
| |
| 作者单位： | 1. 上海第二医科大学上海市免疫学研究所,上海,200025
2. 上海第二医科大学医用试剂实验室 |
| |
| 基金项目： | “九五”国家科技攻关计划资助 |
| |
| 摘 要： | 新鲜甲状腺组织经硫酸铵沉淀和两次ＳｅｐｈａｄｅｘＧ－２００凝胶层析后，可获得较高纯度的甲状腺蛋白。经ＳＤＳ－ＰＡＧＥ鉴定，显示了３条区带，主带为１９Ｓ，另两条为２７Ｓ和１２Ｓ。经双向琼脂扩散和免疫电泳鉴定，提纯的甲状腺球蛋白未见血清蛋白质的污染。
|
| 关 键 词： | 甲状腺球蛋白 纯化 鉴定 盐析 Sephadex G-200 |
PURFICATION AND IDENTICATION OF HUMAN THYROGLOBULIN |
| |
| Chen Guangjie,Xi Yebin,Wang Baoguo,Zhou Wenda,Li Weiyi. PURFICATION AND IDENTICATION OF HUMAN THYROGLOBULIN[J]. Immunological Journal, 1999, 15(1): 61-63 |
| |
| Authors: | Chen Guangjie Xi Yebin Wang Baoguo Zhou Wenda Li Weiyi |
| |
| Abstract: | Thyroglobulin(TG) was extracted from fresh thyroid tissues removed from thyrophymaic patients with ammonium sulfate precipitation and purified by repeated gel filtration on Sephadex G 200. The presence of TG in the main peak of Sephadex G 200 fractions was identified by SDS polyacrylamide gel electrophoresis, showing 3 bands which represent 19S, 27S and 12S components, but 19S was the main components. |
| |
| Keywords: | Thyroglobulin Purification Identification Salting out Sephadex G 200 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
