尤瑞克林对脑缺血再灌注大鼠脑组织中IGF-1和IGF-1R表达水平的影响及其机制

目的:观察尤瑞克林对大鼠脑缺血再灌注损伤后缺血区脑组织中胰岛素样生长因子1(IGF-1)及其受体(IGF-1R)表达的影响,分析尤瑞克林的脑保护机制。方法:24只成年SD大鼠随机分为假手术组(n=8)、模型组(n=8)和实验组(n=8)。以线栓法制作大鼠大脑中动脉脑缺血再灌注模型,假手术组大鼠接受相似手术处理,但是不插入线栓,实验组大鼠再灌注后5 min给予尤瑞克林,假手术组和模型组正常喂养。在缺血2 h再灌注48 h后断头取脑,采用RT-PCR、免疫组织化学和Western blotting法检测各组大鼠缺血区脑组织中IGF-1和IGF-1R mRNA和蛋白的表达及阳性细胞数。结果:与假手术组比较,模型组和实验组大鼠缺血区脑组织中IGF-1和IGF-1R mRNA和蛋白的表达水平及阳性细胞数明显升高(P<0.05);与模型组比较,实验组大鼠缺血区脑组织中IGF-1和IGF-1R mRNA和蛋白的表达水平及阳性细胞数明显升高(P<0.05)。结论:尤瑞克林可改善脑缺血再灌注,其机制可能与促进IGF-1和IGF-1R的表达有关联。

Effects of urinary kallidinogenase on expression levels of IGF-1 and IGF-1R in brain tissue of rats with cerebral ischemia reperfusion and their mechanisms

Objective To observe the effect of urinary kallidinogenase on the expressions of insulin-like growth factor 1(IGF-1) and insulin-like growth factor 1 receptor(IGF-1R) after ischemia reperfusion injury in the rats, and to clarify the mechanism of brain protection.Methods 24 adult SD rats were randomly divided into sham operation group(n=8), model group(n=8),and experiment group(n=8).The middle cerebral artery occlusion reperfusion models were made by the suture method for the rats in model and experiment groups.The rats in sham operation group were taken the similar operation without suture.The rats in model group were given urinary kallilidinogenase 5 min after the reperfusion.The expressions of IGF-1 and IGF-1R mRNA and proteins,and the number of IGF-1 and IGF-1R positive cells in the ischemic region of the brain tissue of the rats in various groups were detected by RT-PCR,immunohistochemical,and Western blotting methods.Results Compared with sham operation group,the expression levels of IGF-1 and IGF-1R mRNA and proteinsand the number of positive cells in the brain tissue of the rats in model group and experiment group were increased (P<0.05);compared with model group,the expression levels of IGF-1 and IGF-1R mRNA and proteins and the number of positive cells in the brain tissue of the rats in experimental group were increased(P<0.05).Conclusion Urinary kallidinogenase can improve the cerebral ischemia reperfusion,which is associated with the promotion of the expressions of IGF-1 and IGF-1R.