丹参磷脂浸膏对大鼠脑缺血再灌注损伤的保护作用及其机制

目的:观察丹参磷脂浸膏(DSLZ)对脑缺血再灌注损伤的保护作用,探讨其可能机制。方法:60只Wistar大鼠随机分为假手术组、模型组、阳性对照丹参酮ⅡA组和低、中、高剂量DSLZ组(5.0、10.0、20.0 mg·kg^-1,以丹参酮ⅡA计),每组10只。采用线栓法制备大鼠局灶性脑缺血再灌注损伤模型,缺血2 h、再灌注22 h后检测大鼠神经功能评分,TTC染色检测大鼠脑梗死面积百分比,HE染色检测大鼠脑组织病理形态学,检测大鼠脑组织匀浆中超氧化物歧化酶(SOD)活性、丙二醛(MDA)及一氧化氮(NO)水平。结果:与假手术组比较,模型组大鼠神经功能评分升高(P<0.05),脑梗死面积百分比增大(P<0.05),脑组织中SOD活性明显降低(P<0.01),MDA和NO水平明显升高(P<0.01)。与模型组比较,中、高剂量DSLZ组大鼠神经功能评分降低,脑梗死面积百分比明显缩小,脑组织中 SOD活性明显升高, MDA及NO水平明显降低(P<0.05或 P<0.01)。HE染色,模型组大鼠神经细胞胞膜不清,细胞出现肿胀、变形、坏死,细胞间质水肿,细胞间隙增大;高剂量DSLZ组大鼠神经细胞核仁清晰,间质轻微水肿。结论:DSLZ可减轻脑缺血大鼠神经细胞损伤,其机制可能与减轻自由基损伤、抑制NO神经毒性有关联。

Protective effect of Danshenlinzhi on cerebral ischemia reperfusion injury in rats and its mechanism

Objective To investigate the protective effect of Danshenlinzhi(DSLZ) on the cerebral ischemia reperfusion injury in the rats, and to explore its mechanism.Methods 60 Wistar rats were randomly divided into sham group,model group,TanⅡA group, and low,middle,high, doses (5.0,10.0,20.0 mg·kg^-1) of DSLZ groups(n=10). The thread embolism was performed to establish the rat models of focal cerebral ischemia reperfusion injury. The neurologic scores of the rats after 2 h ischemia and 22 h reperfusion were measured and the percentages of the cerebral infarction area of the rats were detected by TTC staining method; the pathomorphology of the brain tissue of the rats was detected by HE staining. The activities of superoxide dismutase (SOD) and levels of malondialdehyde(MDA),nitric oxide (NO) in the brain tissue of the rats were detected.Results Compared with sham group,the neurologic score of the rats in model group was increased(P<0.05),the percentages of the cerebral infarction area was increased(P<0.05),the activity of SOD was decreased(P<0.01) and the levels of MDA and NO were increased (P<0.01). Compared with model group,the neurologic scores of the rats in middle and high doses of DSLZ groups were decreased and the percentages of the cerebral infarction area were decreased,the activities of SOD were increased and the levels of MDA and NO were decreased(P<0.05 or P<0.01).The HE staining results showed the membrane of neurocyte was unclear,and the cells became swelling,deformation or necrosis,severe edema in interstitium and the cell space was enlarged in model group;the nucleolus could be found clearly and there was slight edema in interstitium of the cells of the rats in high dose of DSLZ group.Conclusion DSLZ has a protective effect on the cerebral ischemia reperfusion injury of the rats,and its mechanism may be related to the anti-radical damage and lessening the neurotoxicity of NO.