胸水细胞蜡块结合免疫组化学技术在晚期肺腺癌中的应用

目的 分析胸水细胞蜡块结合免疫组织化学在肺腺癌中的应用。方法 收集东阳市中医院2008年6月~2015年12月 7年间50例可疑恶性胸水样本，分析其临床病史、涂片、细胞蜡块切片、免疫组织化学及基因检测等资料。结果 50例可疑恶性胸水患者，增生反应性间皮细胞4例，确诊恶性肿瘤46例，其中肺腺癌27例，其他组织来源及组织类型19例，与细胞学常规涂片相比，细胞蜡块切片镜下可见背景干净，染色对比清晰，细胞数目明显增多、厚薄均匀，且可以寻找组织来源及基因检测。结论 细胞蜡块结合免疫组织化学技术极大地提高了胸水细胞学的诊断准确率及明确组织来源；同时为肿瘤精准医疗提供了平台，值得推广。

Application of pleural effusion cell wax block combined with immunohistochemistry in advanced lung adenocarcinoma

Objective To analyze the application of pleural effusion cell wax block combined with immunohistochemistry in lung adenocarcinoma. Methods 50 cases of suspected malignant pleural effusion from June 2008 to Decembe 2015 were collected from Dongyang Traditional Chinese Hospital. The clinical history, smears, cell wax block,immunohistochemistry and gene detection were analyzed. Results There were 4 cases of proliferative reactive mesothelial cells and 46 cases of diagnosed malignant tumors in 50 cases of suspected malignant pleural effusion. Among them, 27 cases were lung adenocarcinoma and 19 cases were from other tissue sources and tissue types. Compared with that of cytological routine smears, the background of cell wax block was clean under the microscope, and the staining contrast was clear; the number of cells was obviously increased; the thickness was even; the tissue source and gene detection could be found. Conclusion The cell wax immunohistochemistry technique greatly improves the diagnostic accuracy of pleural effusion cytology and clarifies the source of tissue. At the same time, it provides a platform for accurate tumor treatment and is worthy of promotion.