Abstract: | Objective To understand the anti-HIV antibody and HIV-RNA detection of unpaid blood donors in Yangzhou City from February 2015 to December 2017.Methods?Anti-HIV antibodies were detected by two-pass ELISA in 121,617 unpaid blood donors from February 2015 to December 2017 in Yangzhou City.Then,the HIV-RNA of 116,500 nucleic acid samples with both-sided negative or one-sided negative ELISA results were detected by PCR.The anti-HIV antibody positive samples were sent to Yangzhou CDC for confirmation,and the HIV-RNA positive samples were sent to the Jiangsu Provincial Blood Center for further confirmation.Results?57 cases of positive anti-HIV antibodies from February 2015 to December 2017 accounted for 0.05%.And 13 were positive,accounting for 0.01%.Confirmed positive specimens were ELISA double reagent positive specimens.Single reagent positive specimens by ELISA method were negative after confirmation.There were 4 cases of positive HIV-RNA,accounting for 0.003%,and 2 cases of confirmed positive,accounting for 0.002%.Conclusion?Relevant departments of blood collection and blood supply institutions should fully consider the distribution characteristics of HIV infection in the population and adjust the strategy when consulting for recruitment before donating blood.Relevant departments should strengthen the promotion and popularization of HIV-related knowledge to further ensure blood safety.In the detection of blood virus,nucleic acid detection and enzyme-linked immunosorbent assay have their own advantages,and the two complement each other,and the two are combined to improve the accuracy of blood virus detection. |