Routine Determination of GFR in Renal Transplant Recipients by HPLC Quantification of Plasma Iohexol Concentrations and Comparison With Estimated GFR |
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Authors: | Stéphanie Castagnet Hélène Blasco Patrick Vourc'h Isabelle Benz‐De‐Bretagne Charlotte Veyrat‐Durebex Christelle Barbet Azmi Alnajjar Bénédicte Ribourtout Matthias Buchler Jean‐Michel Halimi Christian R Andres |
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Institution: | 1. CHRU de Tours, Laboratoire de Biochimie et Biologie Moléculaire, , Tours, France;2. UMR INSERM U930, CNRS ERL 3106, Université Fran?ois Rabelais de Tours, , Tours, France;3. CHRU de Tours, Service de Néphrologie‐Immunologie clinique, , Tours, France;4. EA 4245, Université Fran?ois Rabelais de Tours, , Tours, France |
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Abstract: | Estimated glomerular filtration rate (eGFR) methods are not sufficiently reliable in renal transplant recipients (RTR) and should be replaced by iohexol plasma clearance measurement. However, this method has poor availability in health centers. The aim of our study was to develop a high‐performance liquid chromatography (HPLC) method for plasma iohexol measurement in routine practice and to evaluate its plasma clearance as a reference of GFR. We developed an HPLC method using UV detection. We evaluated sample storage conditions to provide recommendations for routine practice. Then, we compared GFRbased on plasma iohexol clearance (GFR‐iohexol) to eGFR using modification of diet in renal disease, Cockcroft and Gault, and CDK‐EPIequations in 40 RTR. The method was validated over a concentration range of 15–300 μg/l. Excellent linearity (r > 0.998), inter‐ and intraday precision (CV < 3.3%), and accuracy (>96.8%) were complied with ICH guidelines. We also demonstrated excellent samples stability (9 days). Although eGFR methods are not references in RTR, we found a correct concordance between eGFR and GFR‐iohexol in our population. To conclude, our method is simple, rapid, accurate, and reliable for routine clinical and research use especially in RTR. J. Clin. Lab. Anal. 26:376‐383, 2012. © 2012 Wiley Periodicals, Inc. |
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Keywords: | high‐performance liquid chromatography iohexol renal clearance GFR renal transplant recipients |
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