| 新等位基因HLA-DRB1＊1462的核苷酸序列分析 |
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| 引用本文： | 崔爽,单小燕,何小枚,刘娜,王琳,李伟,王丽君,倪蕾,王东梅,赵波涛,龚治尹,张志欣. 新等位基因HLA-DRB1＊1462的核苷酸序列分析[J]. 中国实验血液学杂志, 2009, 17(2): 493-495 |
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| 作者姓名： | 崔爽 单小燕 何小枚 刘娜 王琳 李伟 王丽君 倪蕾 王东梅 赵波涛 龚治尹 张志欣 |
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| 作者单位： | 北京市红十字血液中心HLA实验室,北京,100088 |
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| 摘 要： | 本研究验证一个新的HLA等位基因DRB1＊1462的核苷酸序列。应用MR-SSO和MR-SSP基因分型技术对HLA分型，对怀疑是HLA新等位基因进行DNA测序。结果发现，该序列与已知的所有HLA-DRB1等位基因序列不一致，与HLA—DRB1＊140101的序列差异仅表现在第2外显子区域中256位碱基发生了G-〉A的替换，导致相应的氨基酸由丙氨酸变为苏氨酸。结论：该基因是HLA-DRB1位点的一个新等位基因，已经被WHOHLA命名委员会于2006年1月30日正式命名为HLA-DRB1女1462。
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| 关 键 词： | HLA等位基因 HLA—DRB1＊1462 SBT 序列分析 |
Identification of a Novel HLA Allele DRB1*1462 by Sequence-Based Typing |
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| CUI Shuang,SHAN Xiao-Yan,HE Xiao-Mei,LIU Na,WANG Lin,LI Wei,WANG Li-Jun,NI Lei,WANG Dong-Mei,ZHAO Bo-Tao,GONG Zhi-Yin,ZHANG Zhi-Xin. Identification of a Novel HLA Allele DRB1*1462 by Sequence-Based Typing[J]. Journal of experimental hematology, 2009, 17(2): 493-495 |
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| Authors: | CUI Shuang SHAN Xiao-Yan HE Xiao-Mei LIU Na WANG Lin LI Wei WANG Li-Jun NI Lei WANG Dong-Mei ZHAO Bo-Tao GONG Zhi-Yin ZHANG Zhi-Xin |
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| Affiliation: | (HLA Laboratory, Beijing Red Cross Blood Center, Beijing 100088, China) |
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| Abstract: | This study was to identify a novel HLA-DRB1 allele in Chinese population by nucleotide sequence analysis. The HLA typing of genes was performed by PCR-SSO and PCR-SSP, the ambiguous novel allele was identitied by DNA sequence analysis. The results showed that the sequence of this new allele differed from DRB1 ＊ 140101 by one nucleotide substitution at position 256 in exon 2 （G - 〉 A）, resulting in an amino acid change from Ala to Thr at codon 57. In conclusion, this allele is a novel one, which has been officially given the name DRB1 ＊ 1462 by the WHO nomenclature committee in January 2006. |
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| Keywords: | HLA-DRB1*1462 SBT |
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