Elevated peroxidative glutathione redox status in atherosclerotic patients with increased thickness of carotid intima media |
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作者单位: | HUANG Yan-sheng,WANG Li-xia,SUN Lin,WU Yu,LU Jian-min,ZHAO Shi-chao,DAI Fu-min,XU Bo-shi(Department of Cardiology, Henan Provincial People's Hospital,Zhengzhou, Henan 450003, China);WANG Shu-ren(Department of Pathophysiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, China) |
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基金项目: | Acknowledgements: We are grateful to the staff of the Department of Cardiology, Henan Provincial People's Hospital and Department of Pathophysiology, West China School of Preclinical and Forensic Medicine, Sichuan University. We are also grateful to Professor WEI Tai-xiug and Professor WEI Jing-han for their advice to the study. |
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摘 要: | Background Atherosclerosis is a chronic inflammatory disease. Accumulated evidences suggest a deep involvement of oxidative damage in the development of atherosclerosis, but little is discussed over the relationship between plasma glutathione redox status as the most important intrinsic antioxidant defensive mechanism and the atherosclerosis. Methods A total of 132 patients suspected with atherosclerosis were assigned to three groups by high frequency ultrasonic examination of the carotid artery. With the thickness of intima of the carotid artery as an index of degree of atherosclerosis progression, 56 were included in plaque-forming group (A), 42 in carotid artery intima-thickening group (B), and 34 in normal carotid artery intima-thickness group (C). All patients were subjected to the measurement of plasma glutathione (GSH) (reduced form GSH and oxidized form GSSG), nicotinamide adenine dinucleotide phosphate (NADP) (reduced form NADPH and oxidized form NADP^+), oxidized low density lipoprotein (oxLDL), and malondialdehyde (MDA) The GSH/GSSG and NADPH/NADP^+ redox potentials were calculated according to the Nernst equation, and their correlation with intima thickness and oxLDL was analyzed. Results With the thickening of artery intima (from group C to A), GSH concentration and the ratio of GSH/GSSG gradually reduced, and GSSG and GSH/GSSG redox potential gradually increased (more positive) (P 〈0.05). The NADPH and NADPH/NADP^+ redox status also showed similar but milder changes. The products of oxidative stress oxLDL and MDA increased significantly along with the thickening of artery intima (P 〈0.05). The analysis of the relationship between GSH/GSSG redox potential, intima thickness, and oxLDL showed positive correlations (P 〈0.05). The plasma GSH/GSSG redox status was positively correlated with the intima thickness of the carotid artery and the oxidized injury of LDL. The redox status shifted to oxidizing direction along with the intima thickening and plaque-forming. Conclusion Elevated peroxidative glutathione redox status was deeply implicated in atherosclerosis progressing, and it may be a sensitive and reliable index for monitoring oxidative status in atherosclerosis.
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关 键 词: | atherosclerosis redox status glutathione nicotinamide adenine dinucleotide phosphate low density lipoprotein oxidized |
Elevated peroxidative glutathione redox status in atherosclerotic patients with increased thickness of carotid intima media |
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Authors: | HUANG Yan-sheng WANG Li-xia SUN Lin WU Yu LU Jian-min ZHAO Shi-chao DAI Fu-min XU Bo-shi WANG Shu-ren |
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Institution: | HUANG Yan-sheng(Department of Cardiology, Henan Provincial People's Hospital,Zhengzhou, Henan 450003, China);WANG Li-xia(Department of Cardiology, Henan Provincial People's Hospital,Zhengzhou, Henan 450003, China);SUN Lin(Department of Cardiology, Henan Provincial People's Hospital,Zhengzhou, Henan 450003, China);WU Yu(Department of Cardiology, Henan Provincial People's Hospital,Zhengzhou, Henan 450003, China);LU Jian-min(Department of Cardiology, Henan Provincial People's Hospital,Zhengzhou, Henan 450003, China);ZHAO Shi-chao(Department of Cardiology, Henan Provincial People's Hospital,Zhengzhou, Henan 450003, China);DAI Fu-min(Department of Cardiology, Henan Provincial People's Hospital,Zhengzhou, Henan 450003, China);XU Bo-shi(Department of Cardiology, Henan Provincial People's Hospital,Zhengzhou, Henan 450003, China);WANG Shu-ren(Department of Pathophysiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, China); |
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Abstract: | Background Atherosclerosis is a chronic inflammatory disease. Accumulated evidences suggest a deep involvement of oxidative damage in the development of atherosclerosis, but little is discussed over the relationship between plasma glutathione redox status as the most important intrinsic antioxidant defensive mechanism and the atherosclerosis. Methods A total of 132 patients suspected with atherosclerosis were assigned to three groups by high frequency ultrasonic examination of the carotid artery. With the thickness of intima of the carotid artery as an index of degree of atherosclerosis progression, 56 were included in plaque-forming group (A), 42 in carotid artery intima-thickening group (B), and 34 in normal carotid artery intima-thickness group (C). All patients were subjected to the measurement of plasma glutathione (GSH) (reduced form GSH and oxidized form GSSG), nicotinamide adenine dinucleotide phosphate (NADP) (reduced form NADPH and oxidized form NADP~+), oxidized low density lipoprotein (oxLDL), and malondialdehyde (MDA). The GSH/GSSG and NADPH/NADP~+ redox potentials were calculated according to the Nernst equation, and their correlation with intima thickness and oxLDL was analyzed.Results With the thickening of artery intima (from group C to A), GSH concentration and the ratio of GSH/GSSG gradually reduced, and GSSG and GSH/GSSG redox potential gradually increased (more positive) (P<0.05). The NADPH and NADPH/NADP~+ redox status also showed similar but milder changes. The products of oxidative stress oxLDL and MDA increased significantly along with the thickening of artery intima (P<0.05). The analysis of the relationship between GSH/GSSG redox potential, intima thickness, and oxLDL showed positive correlations (P<0.05). The plasma GSH/GSSG redox status was positively correlated with the intima thickness of the carotid artery and the oxidized injury of LDL. The redox status shifted to oxidizing direction along with the intima thickening and plaque-forming. Conclusion Elevated peroxidative glutathione redox status was deeply implicated in atherosclerosis progressing, and it may be a sensitive and reliable index for monitoring oxidative status in atherosclerosis. |
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Keywords: | atherosclerosis redox status glutathione nicotinamide adenine dinucleotide phosphate low density lipoprotein oxidized |
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