麻杏石甘汤防治甲型H1N1流感分子机制的生物信息学分析

目的: 通过构建分子网络及解析生物学通路,探讨麻杏石甘汤防治甲型H1N1流感的分子机制。 方法: 查找麻杏石甘汤中药成分的有效化合物,在PubChem数据库中检索有效成分的靶蛋白,在Gene数据库中查找甲型H1N1流感的相关基因,运用Ingenuity Pathway Analysis (IPA)软件对二者进行构图和解析。 结果: 查找到麻杏石甘汤的靶蛋白186个,甲型H1N1流感相关基因7个。二者的分子网络复杂,生物功能多样,在共同作用的8条生物通路中,有4条通路为细胞免疫相关通路:病毒模式识别受体信号通路、巨噬细胞白介素12(IL-12)信号和生产通路、蛋白激酶(PKR)在干扰素诱导的抗病毒反应中作用通路、肿瘤坏死因子受体I (TNFR1)信号通路。麻杏石甘汤可以调节细胞免疫相关通路中的Toll样受体9(TLR9)、磷脂酰肌醇3-激酶(PI3K)、核苷酸结合的寡聚结构域2受体(NOD2)等多个位点。 结论: 麻杏石甘汤可以通过干预细胞免疫相关通路中的多个位点起到防治甲型H1N1流感的作用。

Bioinformatics Analysis on Molecular Mechanism of Maxing Shigan Decoction in Treating H1N1 Influenza A

Objective:To study the molecular mechanism of Maxing Shigan decoction in the treatment of H1N1 influenza A:by establishing molecular networks and comparing canonical pathways. Method: Target proteins of Maxing Shigan decoction and related genes of H1N1 influenza A were searched based on Pubchem and Gene databases on line. Molecular networks and canonical pathways comparison analyses were performed by Ingenuity pathway Analysis(IPA). Result: There were 186 targets proteins of Maxing Shigan decoction and 7 related genes of H1N1 influenza A. The molecular networks of Maxing Shigan decoction and H1N1 influenza A were multifunction. There were four cellular immune related pathways in all eight common pathways including:role of pattern recognition receptors in recognition of viruses, interlewkin 12(IL-12) signaling and production in macrophages, role of proten kinase(PKR) in interferon induction and antiviral response and Tumor necrosis factor receptor 1(TNFR1) signaling.Maxing Shigan decoction could regulate Toll-like receptor(TLR9), phosphoinostide 3 -kinase(PI3K), nucleotide-binding oligomerization domain 2(NOD2) and other molecules of cellular immune related pathways. Conclusion: Regulating many effective nodes of cellular immune related pathways maybe the way of Maxing Shigan decoction against H1N1 influenza A.