神经生长因子和脑源性神经营养因子在氧化应激诱导细胞凋亡中的作用

目的 探讨神经生长因子（NGF）和脑源性神经营养因子（BDNF）在氧化应激诱导细胞凋亡的作用。方法 脂质体介导入NGF和BDNF基因转染鼠成纤维细胞NIH3T3，使目的基因在细胞中表达，过氧化氢（H2O2）处理转染细胞，吖啶橙荧光染色法和流式细胞检测细胞凋亡。结果 50μmol/LH2O2能诱导NIH3T3细胞凋亡，处理后24h凋亡率为16．7％，而NIH3T3细胞分别经NGF、BDNF和NGF／BDNF转染表达目的基因后，能对抗H2O2诱导的细胞凋亡，其24h凋亡率分别为8．21％、9．51％和6．26％。结论 氧化应激能诱导细胞凋亡，NGF和BDNF能抑制氧化应激诱导的细胞凋亡，两者具有协同作用。

Effects of nerve growth factor and brain-derived neurothophic factor on oxidati ve stress-induced cell apoptosis

Objective　To investigate the effects of nerve growth fact or(NGF) and brain-derived neurothophic factor (BDNF) on oxidative stress-induc ed cell apoptosis. 　Methods　 After transfection of hNGF or/and hBDNF cDNA mediat ed by Lipofect AMINE, fibroblast cells were treated with 50 μmol/L H2O2, an d then , apoptosis of the cells was observed by fluorescence staining with acridine ora nge morphologically and flow cytometry analysis. 　Results　 Apo ptosis of fibroblast cells was induced by 50 μmol/L H2O2 and the rate of ap o ptosis 24 h after treatment was 16.7%. Fibroblast cells expression of exogen ous NGF or/and BDNF was prevented from the H2O2-induced apoptosis, the rate s of apoptosis of the 3 groups (NGF transfection, BDNF transfection and NGF & BD NF cotransfection) were 8.21%, 9.51% and 6.26%, respectively 24 h af ter H2O2 treatment. 　Conclusions　 NGF or/and BDNF can protect fibroblast cells fro m oxidative stress-induced cell death, and there is coordination between NGF an d BDNF in protecting against oxidative stress.